We narrowed to 5,366 results for: pcDNA3.1+

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pcDNA3.1-ERBB4 (kinase dead)

Plasmid

#29533

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-V5H6-mPCSK3 D153A

Plasmid

#122681

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Mutation

changed Aspartic Acid 153 to Alanine

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tras light chain

Plasmid

#216294

Purpose

Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Tras light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pert Light chain

Plasmid

#216310

Purpose

Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Pert Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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mTiam1-mcherry-sspB in pcDNA3.1

Plasmid

#85221

Purpose

Role of Rac selective GEF domain from Tiam1 in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

mTiam1 (1030-1406) (Tiam1 Mouse)
mcherry
sspB

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 T134R-pcDNA3.1

Plasmid

#118211

Purpose

Expresses FLAG-OTUB1 T134R mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed threonine 134 to arginine

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFV1/myc-His

Plasmid

#127490

Purpose

Expresses myc-tagged NDUFV1 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFV1 (NDUFV1 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CoV2-Omicron BA.1 spike

Plasmid

#194602

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

Depositor

Ben Murrell

Article

Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

Insert

CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tau Authentic Alu 10-12V337M

Plasmid

#194169

Purpose

Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-associated protein tau 10-12 V337M

Tags

3X flag

Expression

Mammalian

Mutation

Changed Valine 337 to Methinonine

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mCherry-p115(S240A)

Plasmid

#181997

Purpose

Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115(S240A) (ARHGEF1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Serine 240 changed to alanine in p115.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

Plasmid

#181859

Purpose

Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

cp-PKN-mRuby2-GFP(1-0)

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS2-Spike-D614G

Plasmid

#166850

Purpose

For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

Depositor

Neville Sanjana

Article

Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

Insert

SARS-CoV-2 Spike (S SARS-CoV-2 virus)

Tags

C9

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

Plasmid

#209851

Purpose

Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

Depositor

Volker Busskamp

Article

Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

Insert

light-driven sodium pump ErNaR

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EGFP(W58stop)-HiBiT

Plasmid

#180508

Purpose

Mammalian expression of EGFP(W58stop)-HiBiT

Depositor

Masaharu Somiya

Article

Somiya et al bioRxiv 2022.01.25.477620

Insert

EGFP(W58stop)

Tags

HiBiT

Expression

Mammalian

Mutation

W58(UGG) to amber stop codon(UAG)

Promoter

CMV

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-KLF12/pcDNA3.1-Neo

Plasmid

#81069

Purpose

Expression of epitope-tagged human KLF12 in mammalian cells.

Depositor

Julian Downward

Article

Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

Insert

FLAG-KLF12 (KLF12 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-GFP-MTn-KDEL

Plasmid

#154348

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

GFP

Expression

Mammalian

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5

Plasmid

#112840

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Full length Grb2

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-2-130-FLAG

Plasmid

#35984

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV2 130 amino acids

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-kappa-HA-dL5-BKa

Plasmid

#73212

Purpose

Expresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Pratt et al Bioconjug Chem. 2015 Sep 16;26(9):1963-7

Insert

BKalpha (Kcnma1 Mouse)

Tags

dL5**

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRIPK1-Flag-S320A

Plasmid

#112491

Purpose

Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S320A

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K13R

Plasmid

#63566

Purpose

Expression of human H2A with K13R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 13 is now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K15R

Plasmid

#63567

Purpose

Expression of human H2A with K15R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 15 is now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Myc-huMEKK3 (K391A)

Plasmid

#44157

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

MEKK3 (K391A) (MAP3K3 Human)

Tags

Myc

Expression

Mammalian

Mutation

K391A, kinase-dead mutant

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

Plasmid

#112829

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

Insert

LL5BIP (2410002F23Rik Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-EGFP

Plasmid

#198512

Purpose

Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/NDUFS8/myc-His

Plasmid

#127489

Purpose

Expresses myc-tagged NDUFS8 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS8 (NDUFS8 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-1-delta2-26-FLAG

Plasmid

#35983

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1 aa 2-26 deleted

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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