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Showing: 2551 - 2600 of 2949 results

  4. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

    Plasmid
    #164458
    Purpose
    AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minCMV
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    164458-plasmid-map-sequence-id-325690

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  30. ER2-mhYFP

    Plasmid
    #186528
    Purpose
    Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Monomeric hyperfolder YFP
    Use
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186528-plasmid-map-sequence-id-373452

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  35. CyTERM-mGFP

    Plasmid
    #62237
    Purpose
    ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.
    Depositor
    Erik Snapp
    Article
    Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1
    Insert
    CyTERM-mGFP
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    signal anchor of rabbit cytochrome p450 and monom…
    Promoter
    CMV
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62237-plasmid-map-sequence-id-320621

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  42. pCytERM-mhYFP

    Plasmid
    #186530
    Purpose
    Visualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Monomeric hyperfolder YFP
    Use
    Tags
    Cytochrome p450 first 29 animo acids (Oryctolagus…
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186530-plasmid-map-sequence-id-368240

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  44. ER2-mGreenLantern

    Plasmid
    #186548
    Purpose
    Localization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    mGreenLantern
    Use
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186548-plasmid-map-sequence-id-373442

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  45. ER2-LSSmGFP

    Plasmid
    #186538
    Purpose
    Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP
    Use
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186538-plasmid-map-sequence-id-368236

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  49. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid
    #185687
    Purpose
    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).
    Depositor
    Peter Walter
    Article
    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75
    Insert
    ERmembrane-GST-2xHaloTag-KKMP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    185687-plasmid-map-sequence-id-366415

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Showing: 2551 - 2600 of 2949 results