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GST-tag LUBAC binding E2 variant D3LU-1

Plasmid

#248858

Purpose

N-term GST tag E2 variant selected for LUBAC

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-D3LU-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--PrecA-YFP

Plasmid

#228097

Purpose

recA promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pMPRAv3:minP-GFP

Plasmid

#109036

Purpose

MPRA v3 minimal promoter with GFP donor

Depositor

Pardis Sabeti, Ryan Tewhey

Article

Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

Insert

emGFP

Expression

Mammalian

Promoter

minP

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Gal4DBD2xHighMag1

Plasmid

#182023

Purpose

Donor plasmid for conversion of Gal4 line into ShineGal4 drivers

Depositor

Jean-Paul Vincent

Article

di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

Insert

Gal4DBD2xHighMag1

Use

Donor plasmid

Expression

Insect

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAG364-greenFAST

Plasmid

#160353

Purpose

expresses greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

greenFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAG365-redFAST

Plasmid

#160354

Purpose

Expresses redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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V309 - pDNR MCS SA lacZ alpha - Donor

Plasmid

#11692

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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AeU6 3p3 GFP LgRNA

Plasmid

#182208

Purpose

For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

3xP3-GFP

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-tevs-KKMP V3

Plasmid

#179640

Purpose

TEVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pEF6.HA-ARRDC3

Plasmid

#38317

Depositor

Quan Lu

Article

Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 20

Insert

ARRDC3 (ARRDC3 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pDest-635

Plasmid

#159582

Purpose

Gateway Destination vector for protein expression in baculovirus

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTCAV-FLEx(loxP)-FlpO

Plasmid

#67829

Purpose

Cre-dependent expression of FlpO from CAV2 virus

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

FlpO

Expression

Mammalian

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pQIq_MRGS_HIS8_(27F3)_FLAG

Plasmid

#199615

Purpose

Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27F3

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag PA1

Plasmid

#15560

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pPBpuro-hM3D-P2Av3-mCherryNLS

Plasmid

#184714

Purpose

Gq DREADD

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

hM3D

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSCW02

Plasmid

#72301

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Expression

Bacterial

Promoter

lac

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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p3HB2ALuc

Plasmid

#234808

Purpose

Mammalian expression of human B2-adrenergic receptor fused to RLuc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

B2-adrenergic receptor

Tags

3xHA and RLuc8

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAG308-His-redFAST

Plasmid

#160350

Purpose

Expresses His-tagged redFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-redFAST

Expression

Bacterial

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPd_PepTSt

Plasmid

#58333

Purpose

Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

Simon Newstead

Article

Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

Insert

PepTSt

Tags

GFP-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only