We narrowed to 10,315 results for: plasmids 101

1. pFTtR5

   Plasmid

   #167508

   Purpose

   Broad-Host range expression vector, RSF1010 origin, TtgRAM repressor, PTtg promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   TtgRAM-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PTtg

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFLxTd5

   Plasmid

   #167519

   Purpose

   Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, tdKatushka2, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-tdKatushka2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLuxB

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEU

   Plasmid

   #213846

   Purpose

   LEU integrating plasmid backbone used to create all of the reporter plasmids.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-lambdaN-HA-DmPABPC1-V5His6_G

   Plasmid

   #146368

   Purpose

   Insect Expression of DmPABPC1

   Depositor

   Elisa Izaurralde

   Insert

   DmPABPC1 (pAbp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one non silent mutation G26S, and seven silent mu…

   Promoter

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-DmPABPC1-V5His6_G

   Plasmid

   #146352

   Purpose

   Insect Expression of DmPABPC1

   Depositor

   Elisa Izaurralde

   Insert

   DmPABPC1 (pAbp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one non silent mutation G26S, and seven silent mu…

   Promoter

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFVGe2

   Plasmid

   #149500

   Purpose

   Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, GmR, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   VanR(AM)-GmR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PVanCC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRR5

   Plasmid

   #167506

   Purpose

   Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   RhaS-RhaR-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRG5

   Plasmid

   #149486

   Purpose

   Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   RhaS-RhaR-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-lambdaN-DmPABPC1-dsRNAres-V5His6_O

   Plasmid

   #147069

   Purpose

   Insect Expression of DmPABPC1-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmPABPC1-dsRNAres (pAbp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one non silent mutation G26S and four silent muta…

   Promoter

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-human OCT4 DE-SV40-Luc

    Plasmid

    #52414

    Purpose

    Luciferase reporter plasmid for DE OCT4 enhancer activity

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    Distal element of human OCT4 promoter (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-sACE2-WT(732)-8h

    Plasmid

    #154101

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

    Plasmid

    #171101

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Olig2 S. pyogenes, Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAP3K15 gRNA (BRDN0001147819)

    Plasmid

    #76101

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP3K15

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP3K15 (MAP3K15 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

    Plasmid

    #74102

    Purpose

    plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Xenopus laevis beta-tubulin gene promoter region and 5'UTR
    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAVTRE3_Clover

    Plasmid

    #135179

    Purpose

    AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Inserts

    Clover
    TRE3G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    a variant of GFP

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTS2326

    Plasmid

    #169628

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2317

    Plasmid

    #169624

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. P1m

    Plasmid

    #120934

    Purpose

    MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.

    Depositor

    Richard Murray

    Insert

    J23101
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Anti-Copper ATPase 1 [L60/4R-rat IgG2a] - Chimeric

    Plasmid

    #231823

    Purpose

    Mammalian expression plasmid of anti-Copper ATPase 1 (Menke's disease protein) (Human) rat IgG2a R-mab. Derived from hybridoma L60/4.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Copper ATPase 1 (Menke's disease protein) (Homo sapiens) recombinant mouse monoclonal antibody (ATP7A Chimera: M. musculus (mouse)/R. norvegicus (rat))
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Anti-Kir2.3 K+ channel [N25/35R-rat IgG2a] - Chimeric

    Plasmid

    #231848

    Purpose

    Mammalian expression plasmid of anti-Kir2.3 K+ channel (Human) rat IgG2a R-mab. Derived from hybridoma N25/35.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Kir2.3 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNJ4 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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