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p35S_GFP-EVDinter-GUS

Plasmid

#167123

Purpose

Plant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Publication

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)

Tags

GUS and mGFP5

Expression

Plant

Promoter

CaMV 35S

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE2 mutant

Plasmid

#133309

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

Depositor

Josep Domingo-Domenech

Publication

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE2 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

gccacatgcacc changed to gcAaAaAAcacc

Promoter

eIF3B

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-3A

Plasmid

#114054

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Publication

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3A (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670A…

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-3D

Plasmid

#114055

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Publication

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3D (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670D…

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh-1539

Plasmid

#82432

Purpose

Plasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cells

Depositor

Marina Chekulaeva

Publication

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

hs-nvGW182 chimera

Tags

GST

Expression

Mammalian

Mutation

aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

Promoter

EF1A

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFM034

Plasmid

#40137

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (wild-type) (par-2 )

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

pie-1

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
UbC Puro ABL FRET F72P

Plasmid

#235033

Purpose

Expression of BCR-ABL F72P FRET biosensor

Depositor

Justin Pritchard

Publication

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

F72P

Promoter

human ubiquitin C

Available since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET P112L

Plasmid

#235034

Purpose

Expression of BCR-ABL P112L FRET biosensor

Depositor

Justin Pritchard

Publication

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

P112L

Promoter

human ubiquitin C

Available since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET V73A

Plasmid

#235036

Purpose

Expression of BCR-ABL V73A FRET biosensor

Depositor

Justin Pritchard

Publication

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

V73A

Promoter

human ubiquitin C

Available since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET Y253H

Plasmid

#235037

Purpose

Expression of BCR-ABL Y253H FRET biosensor

Depositor

Justin Pritchard

Publication

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

Y253H

Promoter

human ubiquitin C

Available since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET M244V

Plasmid

#235039

Purpose

Expression of BCR-ABL M244V FRET biosensor

Depositor

Justin Pritchard

Publication

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

M244V

Promoter

human ubiquitin C

Available since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET Y342A

Plasmid

#235040

Purpose

Expression of BCR-ABL Y342A FRET biosensor

Depositor

Justin Pritchard

Publication

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

Y342A

Promoter

human ubiquitin C

Available since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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hsp70I: cPla-mKate2-P2A-EGFP-KDEL

Plasmid

#250915

Purpose

Dual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in zebrafish live tissue after heat shock

Depositor

Philipp Niethammer

Publication

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

cPla2-mKate2-P2A-EGFP-KDEL (pla2g4aa Zebrafish)

Use

D.rerio (zebrafish)

Tags

KDEL on EGFP and mKate2 on cPla2

Promoter

hsp70i

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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CD44v-EC-His (pcDNA3)

Plasmid

#238418

Purpose

Expresses the polyhistidine-tagged extracellular region of CD44v (CD44v-EC-His)

Depositor

Amy Shiu Lee

Publication

Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

Insert

CD44 containing extracellular region of CD44v (CD44 Human)

Tags

His

Expression

Mammalian

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUN1213 - pL0_p16OMT (pro + 5U)

Plasmid

#203890

Purpose

16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Publication

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

16OMT promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI and/or BsaI cut sites for MoClo …

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-499/500R

Plasmid

#203575

Purpose

Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

Depositor

Abhishek Chakraborty

Publication

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

silent point mutations to make resistant shRNA 49…

Promoter

CMV

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Publication

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Publication

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 PSK (745-1235)

Plasmid

#197863

Purpose

Expression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian cells (microtubule-binding domain)

Depositor

Jonathan Morris

Publication

Mitsopoulos et al J Biol Chem. 2003 May 16;278(20):18085-9

Insert

PSK1 (745-1235) (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

The N terminus of PSK1/TAOK2 has been deleted

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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KCNJ15_pcDNA6.2/EmGFP-Bsd

Plasmid

#176981

Purpose

Mammalian expression vector encoding KCNJ15 and EmGFP-Bsd

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

KCNJ15 (KCNJ15 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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