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We narrowed to 12,390 results for: nsf

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Showing: 10421 - 10440 of 12390 results

  2. pAAV-CMV-dSa VP64 Actc1

    Plasmid
    #99691
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    99691-plasmid-map-sequence-id-194530

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  3. pAAV_ACTB CRISPIE

    Plasmid
    #172853
    Purpose
    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Inserts
    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172853-plasmid-map-sequence-id-341002

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  4. RCAS-sgATG7

    Plasmid
    #75228
    Purpose
    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh
    Depositor
    Noor Gammoh
    Article
    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:
    Insert
    Autophagy Related 7 (Atg7 Mouse)
    Use
    Retroviral
    Promoter
    U6
    Available Since
    June 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

    Plasmid
    #154779
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    LaG17-SynNotch
    Tags
    LaG17
    Expression
    Mammalian
    Mutation
    204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…
    Promoter
    EF1
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154779-plasmid-map-sequence-id-310404

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  16. RON6-bio

    Plasmid
    #47785
    Purpose
    Expresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RON6
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin replaced…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47785-plasmid-map-sequence-id-68879

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  19. BMR1_02g02691-bio-His

    Plasmid
    #108042
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_02g02691 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Insert
    BMR1_02g02691
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis (C termi…
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available Since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108042-plasmid-map-sequence-id-210248

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  20. BMR1_03g01540-bio-His

    Plasmid
    #108043
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_03g01540 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Insert
    BMR1_03g01540
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis (C termi…
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available Since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108043-plasmid-map-sequence-id-213071

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Showing: 10421 - 10440 of 12390 results