20,084 results

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pCAG-GFPd2

Plasmid

#14760

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

EGFPd2

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-PFO

Plasmid

#92109

Purpose

Recombinant expression in E.Coli

Depositor

Feng Shao

Article

Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

Insert

PFO

Tags

6xHis

Expression

Bacterial

Mutation

Delta27aa

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-EGFP

Plasmid

#114213

Purpose

AAV vector (control) that targets expression of EGFP to neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pQE60-EFG-His

Plasmid

#124126

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor G (fusA Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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AP2u2-mCherry

Plasmid

#27672

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Ap2m1 (Ap2m1 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_LIN28A

Plasmid

#26819

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-LIN28A-3'UTR (LIN28A Human)

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-TGFB1

Plasmid

#116799

Purpose

Lentiviral expression of TGFB1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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GADD45 WT-luc

Plasmid

#8356

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

GADD45 promoter WT (GADD45A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pET N6his GFP1-9

Plasmid

#182240

Purpose

Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

Depositor

Stéphanie Cabantous

Article

Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

Insert

GFP1-9

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-AK1-His

Plasmid

#124135

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Adenylate kinase (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pS-cr:GFP

Plasmid

#196295

Purpose

35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs

Use

CRISPR

Tags

SpeI-DR-BsaI-DR-SpeI

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGreen_dualluc_3'UTR_sensor

Plasmid

#55206

Purpose

Dual-luciferase reporter for microRNA targeting in plants

Depositor

Michael Axtell

Article

Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

Inserts

Renilla luciferase
Firefly luciferase

Use

Luciferase; Plant transient expression

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDH2-WT

Plasmid

#87926

Purpose

Expresses IDH2 (wild-type) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Vimentin-N-18

Plasmid

#55158

Purpose

Localization: Intermediate Filaments, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Vimentin (VIM Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_ALFA-tag-SNAP

Plasmid

#136628

Purpose

Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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SORT1-bio-His

Plasmid

#52024

Purpose

Expresses full-length Sortilin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

SORT1 (SORT1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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All_in_one_CRISPR/Cas9_LacZ

Plasmid

#74293

Purpose

"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

Depositor

Lynne Postovit

Article

Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

Inserts

LacZ-alpha
Cas9
mCherry

Use

CRISPR

Tags

HA

Expression

Bacterial and Mammalian

Promoter

Lac promoter, SV40, and T7 promoter

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFG eIF4G1

Plasmid

#158775

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-CaNAR2

Plasmid

#64729

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2-NES

Tags

Cerulean3, Nuclear export signal (NES), and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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P210 pcDNA3

Plasmid

#27481

Depositor

Warren Pear

Article

He et al Blood. 2002 Apr 15;99(8):2957-68.

Insert

bcr/abl (ABL1, BCR Human)

Expression

Mammalian

Mutation

Contains the complete bcr/abl fusion

Promoter

CMV

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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