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EC71022

Plasmid

#159608

Purpose

Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

loxP-mCherry-HDEL-t35S-loxP

Use

Cre/Lox and Synthetic Biology

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAcBac1-haloTyrRS C6

Plasmid

#141174

Purpose

Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

Depositor

Ryan Mehl

Article

Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

Insert

C6 HaloTyr tRNA synthetase

Use

Lentiviral

Expression

Mammalian

Mutation

L270S, Y271L, N311G, C313T

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP-LIP

Plasmid

#15714

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LIP (CEBPB Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pRSET a sfGCaMP6s

Plasmid

#100022

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

sfGCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Mutation

cpEGFP replaced with superfolder cpGFP

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

Plasmid

#104122

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

spine-jRGECO1a-P2A-QuasAr-dark Citrine

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Rac1 G15A

Plasmid

#69355

Purpose

expression of nucleotide free Rac1 in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 NSP16

Plasmid

#158367

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP16 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pUAST-GFP-RpL10Ab

Plasmid

#71729

Purpose

Expression of Drosophila melanogaster Rpl10Ab-GFP fusion

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Tags

EGFP

Expression

Insect

Promoter

hsp70

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
EC71173

Plasmid

#154061

Purpose

Level 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-Cre

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

Cre recombinase

Expression

Bacterial

Mutation

Introduction of U5 intron sequences, to prevent p…

Promoter

HvHSP17

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-YdLight1

Plasmid

#125705

Purpose

Expresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Insert

YdLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-TTF-1-gRNA

Plasmid

#101857

Purpose

Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

gRNA targeting TTF-1 (NKX2-1 Human)

Use

Lentiviral

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TASL(FL)

Plasmid

#221263

Purpose

Expression of TASL in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TASL (Tasl Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf8

Plasmid

#158377

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf8 (ORF8 )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf7a

Plasmid

#158375

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7a (ORF7a )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLP-pTac-Tn5-chlorR

Plasmid

#199994

Purpose

Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Landing Pads

Expression

Bacterial

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA-Spec-yibDp-Erv1p

Plasmid

#212094

Purpose

Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

Sulfydryl oxidase (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mIR-31 sponge

Plasmid

#22694

Purpose

Stable suppression of miR-31 function

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

miR-31 (MIR31 Human)

Use

Retroviral

Tags

*

Expression

Mammalian

Mutation

miR-31 sponge with 7x tandem binding sites to the…

Available Since

March 11, 2010

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shLKB1-Hu

Plasmid

#61243

Purpose

Entry clone encoding shRNA to human LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

LKB1 (STK11 Human)

Use

Entr clone

Promoter

H1/TO

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pISceI Dusp6:memGFP

Plasmid

#32668

Purpose

Expresses membrane localized EGFP under the Dusp6 promoter

Depositor

Michael Tsang

Article

Wang et al Development. 2011 Jan;138(1):45-54. Epub

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pKeratin-miRFP670nano

Plasmid

#127437

Purpose

Keratin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

Keratin-miRFP670nano

Tags

miRFP670nano

Expression

Mammalian

Mutation

Keratin V282L - please see depositor comments bel…

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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