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Showing: 10461 - 10480 of 17970 results

  1. pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

    Plasmid
    #172535
    Purpose
    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance
    Depositor
    Richard White
    Article
    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:
    Insert
    -3.5ubb:plin2-tdtomato
    Mutation
    S80R, A81S, E144D, I148F and R415H mutations obse…
    Promoter
    zebrafish ubb
    Available Since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172535-plasmid-map-sequence-id-342927

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  3. NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 PRF

    Plasmid
    #163317
    Purpose
    Encodes for first COVID protein - NSP1, NSP10, NSP11, and fragment of NSP12 fused to nano-luciferase
    Depositor
    Dawn Chandler
    Insert
    Nsp1, Nsp10, Nsp11 ,Nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Luciferase
    Tags
    NanoLuc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    163317-plasmid-map-sequence-id-328584

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  5. pTmpKmH207in

    Plasmid
    #176230
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176230-plasmid-map-sequence-id-347005

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  6. pTmpKmH207out

    Plasmid
    #176231
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176231-plasmid-map-sequence-id-347006

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  7. pTmpKmA1O4in

    Plasmid
    #176232
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176232-plasmid-map-sequence-id-347007

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  8. pTmpKmA1O4out

    Plasmid
    #176233
    Purpose
    A template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176233-plasmid-map-sequence-id-347008

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  12. pNHEJ-RPG

    Plasmid
    #85931
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85931-plasmid-map-sequence-id-172473

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  14. pYFAC-CH3

    Plasmid
    #168979
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Available Since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168979-plasmid-map-sequence-id-350204

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  18. c3GIC9n

    Plasmid
    #62192
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Article
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Insert
    humanized S. Pyogenes Cas9 (D10A)
    Use
    CRISPR
    Tags
    HA
    Mutation
    D10A substitution (Cas9n)
    Promoter
    TRE3G
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    62192-plasmid-map-sequence-id-156364

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  19. lentiMutB3GALT5-blast

    Plasmid
    #208381
    Purpose
    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nucleotide change in PAM targeting sequence nt 51…
    Promoter
    EF-1 alpha core promoter
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208381-plasmid-map-sequence-id-414093

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  20. pUBC_HA_PP7-mCherry

    Plasmid
    #107605
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    PP7-mCherry
    Use
    Synthetic Biology
    Tags
    mCherry
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    hUBC
    Available Since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    107605-plasmid-map-sequence-id-207484

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Showing: 10461 - 10480 of 17970 results