We narrowed to 24,060 results for: crispr
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Plasmid#112295PurposeCRISPR donor plasmid to tag human transcription factor NR4A1 with GFPDepositorInsertNR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
UseCRISPRMutationhg38:12:52058840:C>T, hg38:12:52058930:A>C,…Available SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEY1-donor
Plasmid#112297PurposeCRISPR donor plasmid to tag human transcription factor HEY1 with GFPDepositorInsertHEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
UseCRISPRMutationhg38:8:79765311:G>AAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huMb2Cas12a
Plasmid#174676PurposeExpresses 6xHis-MBP tagged Mb2Cas12aDepositorArticleInserthuMb2Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huTsCas12a
Plasmid#174687PurposeExpresses 6xHis-MBP tagged TsCas12aDepositorArticleInserthuTsCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXT130_TwinStrep-SUMO-ShTnsC
Plasmid#135526PurposeProtein expression construct for ShTnsCDepositorInsertShTnsC
TagsTwinStrep-SUMOExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
BCJJ125
Plasmid#117515PurposeCas9_3 (with intron) Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_3(intron):GCTT:BsaI
UseCRISPRTags2xFLAG and NLSExpressionPlantAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXT131_TwinStrep-SUMO-ShTniQ
Plasmid#135527PurposeProtein expression construct for ShTniQDepositorInsertShTniQ
TagsTwinStrep-SUMOExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-8x-gRNA-eGFP
Plasmid#60718PurposeeGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activationDepositorInsert8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
Available SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXT129_TwinStrep-SUMO-ShTnsB
Plasmid#135525PurposeProtein expression construct for ShTnsBDepositorInsertShTnsB
TagsTwinStrep-SUMOExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX458-HypaCas9
Plasmid#190604PurposePX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1
Plasmid#75388PurposesgRNA1DepositorInsertsgRNA1
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6Available SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huMb3Cas12a
Plasmid#174677PurposeExpresses 6xHis-MBP tagged Mb3Cas12aDepositorArticleInserthuMb3Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huBsCas12a
Plasmid#174671PurposeExpresses 6xHis-MBP tagged BsCas12aDepositorArticleInserthuBsCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…ExpressionBacterialPromoterT7Available SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA
Plasmid#218710PurposeAAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoterDepositorInsertshSaCas9
Chimeric guide for SaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX854
Plasmid#62886PurposeC-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).DepositorInsertSpCas9 (aa573-1368)
UseCRISPRTagsFKBP12, NLS SV40, and NLS nucleoplasminExpressionMammalianPromoterCBhAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
bRA66
Plasmid#100952PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9-HN
Plasmid#104171PurposeCas9-WT mammalian expressionDepositorInsertCas9-WT
UseCRISPRTagsHAExpressionMammalianMutationWTPromoterCMVAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-sgRNA(siteA)
Plasmid#154831PurposesgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cellsDepositorInsertsgRNA(siteA)
UseCRISPRExpressionMammalianPromoterU6Available SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-HDAC1-H141A-Hygro
Plasmid#104417Purposetransient expression of dCas9-dHDAC1 fusion proteinDepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2_8xCTS-ECFP
Plasmid#200249PurposeZebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorInsert8xCTS-ECFP
UseZebrafish expressionAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only