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Showing: 10461 - 10480 of 10500 results

  1. pMCh-1538

    Plasmid
    #82431
    Purpose
    Plasmid for expression of NHA-tagged human-Nematostella GW182 11W11A chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 11W11A chimera
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    CMV
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82431-plasmid-map-sequence-id-156186

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  4. ptE1347D

    Plasmid
    #248055
    Purpose
    Plant organelle-genome-specific random C-to-T mutagenesis
    Depositor
    Shin-ichi Arimura
    Article
    Kosaka et al bioRxiv 2025.11.01.685978
    Insert
    Arabidopsis thaliana RPS5A promoter
    Expression
    Plant
    Mutation
    E1347D mutation in cytidine deaminase domain, low…
    Available Since
    June 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    248055-plasmid-map-sequence-id-496100

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  5. mtE1347D

    Plasmid
    #248057
    Purpose
    Plant organelle-genome-specific random C-to-T mutagenesis
    Depositor
    Shin-ichi Arimura
    Article
    Kosaka et al bioRxiv 2025.11.01.685978
    Insert
    Arabidopsis thaliana RPS5A promoter
    Expression
    Plant
    Mutation
    E1347D mutation in cytidine deaminase domain, low…
    Available Since
    June 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    248057-plasmid-map-sequence-id-496102

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  6. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

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  7. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

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  8. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid
    #191099
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet-I
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    optimized to human codon usage
    Promoter
    UbC
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191099-plasmid-map-sequence-id-380113

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  9. EcNGsC-DelFSH(307-309)-pKK223

    Plasmid
    #131377
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.
    Depositor
    Martin Horvath
    Article
    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021
    Insert
    EcNGsC MutY chimera DelFSH(307-309)
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available Since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131377-plasmid-map-sequence-id-259231

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  10. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61361-plasmid-map-sequence-id-99794

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  11. ptGSVG

    Plasmid
    #248056
    Purpose
    Plant organelle-genome-specific random C-to-T mutagenesis
    Depositor
    Shin-ichi Arimura
    Article
    Kosaka et al bioRxiv 2025.11.01.685978
    Insert
    Arabidopsis thaliana RPS5A promoter
    Expression
    Plant
    Mutation
    4 amino acid changes (S1326G, G1348S, A1398V, S14…
    Available Since
    June 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    248056-plasmid-map-sequence-id-496101

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  12. mtGSVG

    Plasmid
    #248058
    Purpose
    Plant organelle-genome-specific random C-to-T mutagenesis
    Depositor
    Shin-ichi Arimura
    Article
    Kosaka et al bioRxiv 2025.11.01.685978
    Insert
    Arabidopsis thaliana RPS5A promoter
    Expression
    Plant
    Mutation
    4 amino acid changes (S1326G, G1348S, A1398V, S14…
    Available Since
    June 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    248058-plasmid-map-sequence-id-496103

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  13. pATDt

    Plasmid
    #249499
    Purpose
    Expresses tdTomato – Monodirectional chloroplast expression vector (psbH Rescue) in Chlamydomonas Reinhardtii photosynthetically deficient strain CC4388
    Depositor
    Isabel Desgagné-Penix
    Insert
    tdTomato (red fluorescence)
    Use
    Chlamydomonas reinhardtii chloroplast genome
    Expression
    Bacterial
    Mutation
    tdTomato results from the fusion of two copies of…
    Promoter
    The psbD/ 5′ UTR, a light-activated chloroplast p…
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    249499-plasmid-map-sequence-id-497878

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  14. pART27::CLOMELEON

    Plasmid
    #247988
    Purpose
    Stable expression of the indicator protein CLOMELEON for monitoring chloride concentrations in the cytoplasm of plant cells.
    Depositor
    Christoph Plieth
    Article
    Lorenzen et al Plant J. 2004 May;38(3):539-44. doi: 10.
    Insert
    CLOMELEON (CFP-YFP fusion with ENLYFQG-linker)
    Tags
    6xHis
    Expression
    Plant
    Mutation
    CFP-variant: K26R, F64L, S65T, Y66W, N146I, M153T…
    Promoter
    Cauliflower Mosaic Virus 35S promoter (GenBank AJ…
    Available Since
    Jan. 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    247988-plasmid-map-sequence-id-496181

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  15. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

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  16. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  17. mtSepCD_GSVG

    Plasmid
    #248059
    Purpose
    Plant organelle-genome-specific random C-to-T mutagenesis
    Depositor
    Shin-ichi Arimura
    Article
    Kosaka et al bioRxiv 2025.11.01.685978
    Insert
    Arabidopsis thaliana RPS5A promoter
    Expression
    Plant
    Mutation
    4 amino acid changes (S1326G, G1348S, A1398V, S14…
    Available Since
    June 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    248059-plasmid-map-sequence-id-496104

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  18. pBI121::chitRatioGFP:LAAQ

    Plasmid
    #240451
    Purpose
    Expression of indicator protein fusion (modified ratiometric pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of chitinase signal, soluble modified ratiometric pHluorin and low affinity Aequorin (D119A)
    Expression
    Bacterial and Plant
    Mutation
    Ratiometric GFP (ratiometric pHluorin) (PMID 9671…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240451-plasmid-map-sequence-id-485323

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  19. CFP(1-158)-RGS7t

    Plasmid
    #55761
    Purpose
    An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    RGS7(202-477)-CFP(1-158) (RGS7 Human, Aequorea victoria)
    Tags
    CFP(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    The RGS sequence amino terminal to the GGL domain…
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55761-plasmid-map-sequence-id-89566

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  20. CFP(1-158)-gamma-10 in pcDNAI/Amp

    Plasmid
    #55619
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP-(1-158)-gamma-10 (GNG10 Human, Aequorea victoria)
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-10 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55619-plasmid-map-sequence-id-115162

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Showing: 10461 - 10480 of 10500 results