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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 21,629 results for: ESC;

Showing: 10481 - 10500 of 21629 results

  1. pRWJB006

    Plasmid
    #160113
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    35S
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160113-plasmid-map-sequence-id-325470

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  2. pRWJB004

    Plasmid
    #160112
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    UBQ10
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160112-plasmid-map-sequence-id-325469

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  3. pCAG-mEGFP-HO-G

    Plasmid
    #164163
    Purpose
    Expresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminal
    Depositor
    Isei Tanida
    Article
    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1
    Insert
    mEGFP A236K
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    164163-plasmid-map-sequence-id-323957

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  5. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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  6. pCDF-NtXNtS1HR1AtR2NtB2

    Plasmid
    #160890
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-S1H
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-SH1: T7, chaperones: T13
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160890-plasmid-map-sequence-id-318865

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  7. pCDF-NtXNtT4aR1AtR2NtB2

    Plasmid
    #160887
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T4a
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T4a: T7, chaperones: T10
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160887-plasmid-map-sequence-id-318851

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  8. pSWD95Y14D

    Plasmid
    #159098
    Purpose
    CckA FRET Sensor with cyclic-di-GMP point mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated Y514 to D514
    Promoter
    Xylose
    Available since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159098-plasmid-map-sequence-id-310723

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  9. pSWD95D293A

    Plasmid
    #159099
    Purpose
    CckA FRET Sensor with DITE motif mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CckA D293 mutated to A293 and V199I on mRuby3 (P…
    Promoter
    Xylose
    Available since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159099-plasmid-map-sequence-id-316589

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  10. SMT215

    Plasmid
    #134818
    Purpose
    SMT205 with DHFR-FLAG-tag, western blot
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    134818-plasmid-map-sequence-id-313944

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  13. SMT201

    Plasmid
    #134816
    Purpose
    SMT101 with TET promoter for TYMS, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134816-plasmid-map-sequence-id-274005

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Showing: 10481 - 10500 of 21629 results