We narrowed to 4,239 results for: Pet2

1. pET21-pelB-LaM-8

   Plasmid

   #172772

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-8
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28C-mCherry-YTHDF2

   Plasmid

   #204406

   Purpose

   E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-Dnmt3a3L-sc27

   Plasmid

   #71827

   Purpose

   For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

   Depositor

   Albert Jeltsch

   Article

   Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

   Insert

   Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-BCA-Q11

   Plasmid

   #185781

   Purpose

   Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   BCA-Q11
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pet26-MBP-ELIC

   Plasmid

   #39239

   Depositor

   Raimund Dutzler

   Article

   Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

   Insert

   Eriwinia chrysanthemi - pentameric ligand gated ion channel
   

   Use

   Tags

   His-10 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-HaloTag7SS-DogTag

   Plasmid

   #171775

   Purpose

   Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   HaloTag7SS-DogTag
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag inserted in HaloTag7 between residues D139…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-2H12.D11

   Plasmid

   #221145

   Purpose

   His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28_human-pro-IL-18_DeltaN24

   Plasmid

   #214327

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Deletion of first 24 amino acids

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_Caspase-5-enzymatic-domain

   Plasmid

   #214306

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP5 (CASP5 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   aa133-434

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SUMO-AsCas12f

    Plasmid

    #171612

    Purpose

    AsCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-LaM-2

    Plasmid

    #172768

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22b-His6-UBCH7

    Plasmid

    #212711

    Purpose

    Bacterial expression of His6-UBCH7

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    UBCH7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-His-MinD_MinE

    Plasmid

    #133621

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

    Depositor

    Petra Schwille

    Article

    Loose et al Science. 2008 May 9;320(5877):789-92. do

    Insert

    His-MinD_MinE (minD, minE E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET24-ISWI-iCBD

    Plasmid

    #105499

    Purpose

    Expression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)

    Depositor

    James Kadonaga

    Article

    Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

    Insert

    ISWI-iCBD
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-18

    Plasmid

    #172763

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-18
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaG-10

    Plasmid

    #172743

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-10
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-CoilR-Cys

    Plasmid

    #181989

    Purpose

    Used to label proteins with the CoilE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    CoilR-Cys
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaG-42

    Plasmid

    #172758

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-42
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-PTBP1-RRM34

    Plasmid

    #89156

    Purpose

    Recombinant expression of PTBP1-RRM34 in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    contains RNA Recognition Motif 3+4

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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