We narrowed to 2,304 results for: tub

1. pDest27-mBIN1+17

   Plasmid

   #61096

   Purpose

   Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. mCherry-CCHD (p130Cas)

   Plasmid

   #145133

   Purpose

   Expresses CCH domain from p130Cas with an mCherry tag

   Depositor

   Johanna Ivaska

   Article

   Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

   Insert

   p130Cas CCH domain (BCAR1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-POLArISact

   Plasmid

   #164970

   Purpose

   T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

   Depositor

   Sumio Terada

   Article

   Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

   Insert

   POLArISact
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CKAP2-mNeonGreen pHAT

   Plasmid

   #175947

   Purpose

   Bacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagII

   Depositor

   Susanne Bechstedt

   Article

   McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

   Insert

   CKAP2 (Ckap2 Mouse)
   

   Use

   Tags

   6His, StrepTagII, and mNeonGreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C2_3X Flag

   Plasmid

   #194161

   Purpose

   Expresses eGFP fluorescent protein with a 3X flag tag

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   GFP with 3X Flag
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS/U6/RNAi SadBs shRNA mouse

   Plasmid

   #67152

   Purpose

   pBS/U6 containing shRNA to mouse SADBs

   Depositor

   Ana Carrera, Maria Alvarado-Kristensson

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   BRSK1 (Brsk1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. DsRed +9e

   Plasmid

   #191766

   Purpose

   Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Use

   Tags

   N terminal fusion of positively charged polypetod…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDK197

   Plasmid

   #134189

   Purpose

   Retroviral plasmid expressing LAP-Clasp C terminus

   Depositor

   Iain Cheeseman

   Article

   Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

   Insert

   LAP-Clasp C terminus (CLASP1 Human)
   

   Use

   Retroviral

   Tags

   LAP (GFP)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-EHD2b

   Plasmid

   #109569

   Purpose

   Multisite gateway vector for 3' tagging with EHD2b

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   EHD2b (ehd2 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-Climp63(1–301)

    Plasmid

    #186956

    Purpose

    Expresses mCherry-Climp63 with partial luminal domain

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Ckap4 (Ckap4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only remain partial luminal domain

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-Climp63(1–437)

    Plasmid

    #186958

    Purpose

    Expresses mCherry-Climp63 with partial luminal domain

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Ckap4 (Ckap4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only remain partial luminal domain

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CKAP2 pHAT

    Plasmid

    #175946

    Purpose

    Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

    Depositor

    Susanne Bechstedt

    Article

    McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

    Insert

    CKAP2 (Ckap2 Mouse)
    

    Use

    Tags

    6-His and StrepTagII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. myc-Miro1 (lacking TM)

    Plasmid

    #127613

    Purpose

    Human Miro1 with the transmembrane deleted

    Depositor

    Josef Kittler

    Article

    Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Deletion of transmembrane domain

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL2-basic mt E2F-luciferase

    Plasmid

    #133708

    Purpose

    Expression of E2F1-mt-luc.

    Depositor

    James A. DeCaprio

    Article

    Zalvide et al Mol Cell Biol 1998 Mar;18(3):1408-15.

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Trxn factor bind site

    Tags

    Expression

    Mammalian

    Mutation

    the three binding site copies are mutated form

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDK480

    Plasmid

    #134197

    Purpose

    Mammalian expression of Flip in SKAP and IRES GFP

    Depositor

    Iain Cheeseman

    Article

    Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

    Insert

    untagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Alternate Start, G5 siRNA hardened

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E-Dnm2a-DN

    Plasmid

    #109572

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative Dynamin2a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Dynamin2a (dnm2a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMiNGo11-3

    Plasmid

    #126058

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMiNGo11-7

    Plasmid

    #126057

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRa17_1691

    Plasmid

    #49672

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF17_1691
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSmBit-CHIP-K30A

    Plasmid

    #236066

    Purpose

    SmBit-CHIP expression with K30A substitution

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    SmBit-CHIP-K30A (STUB1 Human)
    

    Use

    Luciferase

    Tags

    SmBit

    Expression

    Mutation

    K30A

    Promoter

    HSV-TK

    Available since

    Sept. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRRL-SFFV_V5-CHIC2_IFRPDdel_Tomato

    Plasmid

    #238126

    Purpose

    Expresses V5-tagged CHIC2 (with IFRPD deletion) with Tomato selectable marker.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

    Insert

    V5-tagged CHIC2 with IFRPD deletion
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deleted amino acids 160-165 (IFRPD).

    Promoter

    SFFV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRRL-SFFV_V5-CHIC2-K117R_Tomato

    Plasmid

    #238127

    Purpose

    Expresses V5-tagged CHIC2 (with K117R mutation) with Tomato selectable marker.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

    Insert

    V5-tagged CHIC2 with K117R mutation
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Mutated Lysine 117 to Arginine.

    Promoter

    SFFV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-mCGN

    Plasmid

    #236511

    Purpose

    For expression of GFP-Cingulin (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

    Insert

    CGN (Cgn Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. cCGNL1-HA

    Plasmid

    #236518

    Purpose

    For expression of Paracingulin (dog) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

    Insert

    CGNL1 (CGNL1 Canis lupus familiaris (dog))
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Str-KDEL_SBP-EGFP-PLAP

    Plasmid

    #222308

    Purpose

    Synchronize the trafficking of PLAP from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and PLAP fused to SBP-EGFP (ALPP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p150-CC1-tagBFP

    Plasmid

    #222314

    Purpose

    Inactivates dynein function.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

    Insert

    Coiled-coil domain of p150glued (DCTN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. gRNA giantin/GOLGB1

    Plasmid

    #222315

    Purpose

    CRISPR/Cas9 close to the ATG of giantin/GOLGB1 gene.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

    Insert

    gRNA targeting GOLB1 (GOLGB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. eGFP-SUN1ΔN

    Plasmid

    #213508

    Purpose

    Expresses human eGFP-SUNΔN in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    eGFP-SUNΔN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SUN1ΔN has the first 138 amino acid (lamina domai…

    Promoter

    CMV

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FB399

    Plasmid

    #203635

    Purpose

    Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    FB367+FB396
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FB400

    Plasmid

    #203636

    Purpose

    Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    FB367+FB397
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge