We narrowed to 7,632 results for: ALP
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Plasmid#242420PurposeExpression of the second alpha helix of CHMP6 (residues 57-98), N-terminally tagged with sfGFPDepositorInsertCHMP6 (CHMP6 Human)
TagssfGFP (superfolder GFP)ExpressionMammalianMutationResidues 57-98PromoterCMVAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_P-linker 4E
Plasmid#234564PurposeCTNNA1 Phospholinker phospho-mimicDepositorInsertmEGFP:hCTNNA1_P-linker 4E (CTNNA1 Human)
UseLentiviralTagsmEGFPMutationS641E, S652E, S655E, T658EPromoterCMVAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_P-linker 4A
Plasmid#234563PurposeCTNNA1 Phospholinker phospho-mutantDepositorInsertmEGFP:hCTNNA1_P-linker 4A (CTNNA1 Human)
UseLentiviralTagsmEGFPMutationS641A, S652A, S655A, T658APromoterCMVAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_E307K
Plasmid#234560PurposeCTNNA1 butterfly eye mutantDepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_R551A
Plasmid#234558PurposeCTNNA1 M2-M3 salt-bridge disrupting mutantDepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DNii domain
Plasmid#234557PurposeCTNNA1 Nii-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DNii domain (CTNNA1 Human)
UseLentiviralTagsmEGFPMutationDeletion of aa ~165-265PromoterCMVAvailable SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DM2,3-domain
Plasmid#234556PurposeCTNNA1 M2,3-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DM2,3-domain (CTNNA1 Human)
UseLentiviralTagsmEGFPMutationDeletion of aa 397-509PromoterCMVAvailable SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DM1-domain
Plasmid#234555PurposeCTNNA1 M1-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DM1-domain (CTNNA1 Human)
UseLentiviralTagsmEGFPMutationDeletion of aa 271-396PromoterCMVAvailable SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAPN7 V18D-OSF
Plasmid#193042PurposeMammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
TagsOSF (One-Strep-FLAG)ExpressionMammalianMutationV18DPromoterChicken Beta-actinAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAPN7 L61D-OSF
Plasmid#193043PurposeMammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
TagsOSF (One-Strep-FLAG)ExpressionMammalianMutationL61DPromoterChicken Beta-actinAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAPN7 F98D-OSF
Plasmid#193044PurposeMammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
TagsOSF (One-Strep-FLAG)ExpressionMammalianMutationF98DPromoterChicken Beta-actinAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CAPN7-V18D-mCherry
Plasmid#193046PurposeLentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.DepositorInsertCAPN7 (CAPN7 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationV18D, siRNA resistant to: GCACCCAUACCUUUACAUUPromoterTight TREAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CAPN7-C290S-mCherry
Plasmid#193047PurposeLentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.DepositorInsertCAPN7 (CAPN7 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationC290S, siRNA resistant to: GCACCCAUACCUUUACAUUPromoterTight TREAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 V18D,F98D (1-165)
Plasmid#193034PurposeBacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.DepositorInsertCAPN7 (CAPN7 Human)
Tags6xHis-SumoExpressionBacterialMutationV18D, F98D, Residues 1-165PromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuper Retro GFP DGK alfa human
Plasmid#224578PurposeTo stably downregulate the expression of human DGK alfa in human cellsDepositorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADRA2B-DuET
Plasmid#213178PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SopB(172-554)
Plasmid#183664PurposeN-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expressionDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
TagsEGFPExpressionMammalianMutationAmino acids 172-554PromoterCMVAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SopB(211-554)
Plasmid#183665PurposeN-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expressionDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
TagsEGFPExpressionMammalianMutationAmino acids 211-554PromoterCMVAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C
Plasmid#60394PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptideDepositorInsertTUB1 (TUB1 Synthetic, Budding Yeast)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-198
Plasmid#31065DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseExpressionMammalianMutation-198 to -1 of P2 HNF4A promoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-2dV-Camui (wt)
Plasmid#220366PurposeImproved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)DepositorInsertmVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
TagsmEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)ExpressionMammalianPromoterCAGAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-DmrB-Casp8-2A-mCherry-puro
Plasmid#231971PurposeTet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosisDepositorInsertCasp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)
UseLentiviralAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF0481
Plasmid#142638PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 a-syn A53T D115A mNeonGreen-3K-B11
Plasmid#232017PurposeBacterial expression of alpha synuclein A53T mutant, tagged with the final beta strand of mNeonGreen-3KDepositorAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-MARCer-Blast
Plasmid#160550Purposelabeling hypoxic cellsDepositorAvailable SinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL-Blast FAP S624A
Plasmid#207402PurposeExpresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.DepositorInsertFibroblast Activation Protein Alpha (FAP Human)
UseRetroviralMutationchanged Serine 624 to Alanine. It's a protea…Available SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-Blast FAP A657S
Plasmid#207403PurposeExpresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.DepositorInsertFibroblast Activation Protein Alpha (FAP Human)
UseRetroviralMutationchanged Alanine 657 to Serine. It's an endop…Available SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-A KD/MD
Plasmid#104449PurposeExpression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein A (VAPA Human)
TagsEGFPExpressionMammalianMutationchanged K94 to D and M96 to DAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CRISPRv2-sgIl1r1_#2-hygro
Plasmid#231990PurposeKnockout mouse Il1r1DepositorInsertsgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)
UseCRISPR and LentiviralAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-B KD/MD
Plasmid#104450PurposeExpression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein B (VAPB Human)
TagsEGFP and HAExpressionMammalianMutationchanged K87 to D and M89 to DAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGM238
Plasmid#220178PurposeExpresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)DepositorUseLentiviralTags3xFLAG (NACA); 6xHis (BTF3)MutationK78E (NACA) + K43B (BTF3)Available SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A Δ450-572-pBabe-Puro
Plasmid#25958DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…Available SinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
TOPO-HIF1A
Plasmid#226452PurposeFor subcloning of human HIF1A promoter or for assays using M13 phageDepositorAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF0480
Plasmid#143705PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
APP-bio-His
Plasmid#51643PurposeExpresses full-length Amyloid beta A4 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertAPP (APP Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A/N847A-pBabe-Puro
Plasmid#25956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…Available SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A/N847Q-pBabe-Puro
Plasmid#25948DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…Available SinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pChuk
Plasmid#87033Purposeexpress IKKalpha in mammalian cellsDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA
Plasmid#176460PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRExpressionMammalianPromoterEF1alphaAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Hif1a(776-826)_FIH
Plasmid#99508PurposeBacterial expression of hydroxylated Hif1a TAD (776-826)DepositorTagsHis6GB1ExpressionBacterialPromoterT7Available SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only