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Plasmid#189575PurposeTra synthase (3OC8-HSL)DepositorInsertAcyl-homoserine-lactone synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA2C_Ptet::lasI
Plasmid#189576PurposeLas synthase (3OC12-HSL)DepositorInsertAcyl-homoserine-lactone synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM2
Plasmid#225289PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC311 for pJBM2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM3
Plasmid#225290PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC388 for pJBM3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP6
Plasmid#209961PurposeEntry vector to store Level 0* replication origin partsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSM045
Plasmid#217399PurposeKmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under PtrcDepositorInsertBTE1
UseTagsExpressionBacterialMutationPromoterPTrcAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSM046
Plasmid#217400PurposeKmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under PtrcDepositorInsertBTE2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTrcAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-TEV-AcrIC9
Plasmid#207409PurposeProtein purification vector to produce AcrIC9 in E. coliDepositorInsertT7-TEV-AcrIC9
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-3C-AcrIC8
Plasmid#207410PurposeProtein purification vector to produce AcrIC8 in E. coliDepositorInsertT7-HRV3C-AcrIC8
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gHUS1_B
Plasmid#211542PurposesgRNA-B against HUS1DepositorInsertsgRNA HUS1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgUGP2
Plasmid#211521PurposeDeletes UGP2DepositorInsertsgUGP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-Green Falcan1
Plasmid#199705Purposegreen fluorescent indicator for cAMP imagingDepositorInsertGreen Falcan1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO5
Plasmid#193749PurposeExpresses tdTomato under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selectionDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO3
Plasmid#193748PurposeExpresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selectionDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPAAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO6
Plasmid#193747PurposeExpresses E2-Crimson under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection,DepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR
Plasmid#193746PurposeExpresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertLuxR
UseTagsExpressionBacterialMutationPromoterBBa_J23102Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-HaloGFP-Ca0.5
Plasmid#198632PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-Ca0.5
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-HaloGFP-Ca0.2
Plasmid#198631PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-Ca0.2
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-HaloGFP-Ca1
Plasmid#198633PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-Ca1
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1050U (cinnabar)
Plasmid#132423Purposeexpress arrays of gRNA targeting Cinnabar under dU6-3 promoterDepositorInsertcinnabar gRNA array
UseTagsExpressionInsectMutationPromoterU6-3Available sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050V (wingless)
Plasmid#132424Purposeexpress arrays of gRNA targeting Wingless under dU6-3 promoterDepositorInsertwingless gRNA array
UseTagsExpressionInsectMutationPromoterU6-3Available sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4164342
Plasmid#153553PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsaccatg Kozak-START linkerExpressionMammalianMutationGordon et al recodedPromoterAvailable sinceJune 16, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4164341
Plasmid#153552PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsaccatg Kozak-START linkerExpressionMammalianMutationGordon et al recodedPromoterAvailable sinceJune 16, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTorPE-Y-GECO2m
Plasmid#105066PurposeA yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cellsDepositorInsertY-GECO2m
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-GFP-HA no-pA
Plasmid#82589Purpose3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purificationDepositorInsertGFP-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PM-BAF180-SH3
Plasmid#41933DepositorInsertBAF180
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2s
Plasmid#105067PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.1s
Plasmid#105068PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.1s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pdCas9-bacteria
Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
enz.099-PKA
Plasmid#154048PurposeExpresses PKA in E. coliDepositorInsertProtein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
UseTagsHis, MBPExpressionBacterialMutationPromoterT7Available sinceDec. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-PE_CO-Mini
Plasmid#190338PurposeLentiviral vector expressing PE_CO-Mini (Prime editor with codon-optimized and truncated Reverse transcriptase)DepositorInsertPE_CO-Mini, ngRNA and pegRNA for HEK3 CTTins
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GINKO1
Plasmid#113112PurposeMammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
G-CaMP3
Plasmid#22692Purposea single-wavelength GCaMP2-based genetically encoded calcium indicatorDepositorInsertG-CaMP3
UseTagsExpressionMammalianMutationN363D mutation (increased fluorescence)PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-IRES-Puro
Plasmid#58970Purposenoninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorTypeEmpty backboneUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Clomeleon
Plasmid#203723PurposeA genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentrationDepositorInsertcyan fluorescent protein linked yellow florescent protein
UseTagsExpressionBacterial and MammalianMutationCyan Fluorescent Protein, Topaz Fluorescent Prote…PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-KIRIN1
Plasmid#107416PurposeMammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequenceDepositorInsertKIRIN1
UseTagsNuclear exclusion sequence (NES) (LALKLAGLDIGS)ExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
UseTags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1-mitoRexYFP
Plasmid#60246PurposeMitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.DepositorInsertmitoRexYFP
UseTagsMitochondrial targeting signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA
Plasmid#194247PurposeExpresses 3 miRNAs targeting human alpha-SynucleinDepositorInsertmiRaSynuclein(human) (SNCA Human)
UseAAV and RNAiTagsExpressionMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano65/pcDNA3
Plasmid#51965Purposemammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nMDepositorInsertYellow Cameleon-Nano65
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only