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Showing: 2081 - 2120 of 2761 results

  2. pNHA_SBV_NSs

    Plasmid
    #208932
    Purpose
    Expresses N-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 50 bp from 5' end (including start c…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208932-plasmid-map-sequence-id-419004

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  3. KSP_K560GFP

    Plasmid
    #129770
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129770-plasmid-map-sequence-id-263613

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  5. KSP_K406

    Plasmid
    #129771
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129771-plasmid-map-sequence-id-263572

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  6. BC-GPRC

    Plasmid
    #44199
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    C-terminal fragment of GPR56 starting at amino a…
    Promoter
    LTR
    Available since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44199-plasmid-map-sequence-id-60058

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  7. pSecUAG_UTu1D

    Plasmid
    #233867
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant
    Depositor
    Dieter Söll, Natalie Krahn
    Inserts
    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG) and …
    Promoter
    EM7, araBAD, and native As SelD
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233867-plasmid-map-sequence-id-471093

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  11. pCHA_OROV_NSm

    Plasmid
    #208940
    Purpose
    Expresses C-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSm
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 949 bp from 5' end (Gn), deleted 29…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208940-plasmid-map-sequence-id-419028

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  12. pNHA_OROV_NSm

    Plasmid
    #208935
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSm
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 949 bp from 5' end (Gn), deleted 29…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208935-plasmid-map-sequence-id-419020

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  13. Dronpa-Actin-2

    Plasmid
    #57257
    Purpose
    Localization: Actin, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Use
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    Actin lacks start codon
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. HOXC5 (human) HIS-tag pET

    Plasmid
    #8563
    Depositor
    Corey Largman
    Insert
    HOXC5 (HOXC5 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    Starts at Amino Acid #2 (Ser); makes protein by W…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. pSBtet-Bla/mCardinal-TurboID-GAI

    Plasmid
    #198218
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    mCardinal
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198218-plasmid-map-sequence-id-389373

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  18. pAW32

    Plasmid
    #110225
    Purpose
    integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter
    Depositor
    Tony Carr
    Article
    Watson et al Gene. 2008 Jan 15;407(1-2):63-74
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    110225-plasmid-map-sequence-id-219787

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  19. pAW33

    Plasmid
    #110226
    Purpose
    integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter
    Depositor
    Tony Carr
    Article
    Watson et al Gene. 2008 Jan 15;407(1-2):63-74
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    110226-plasmid-map-sequence-id-212677

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  20. pAW34

    Plasmid
    #110227
    Purpose
    integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter
    Depositor
    Tony Carr
    Article
    Watson et al Gene. 2008 Jan 15;407(1-2):63-74
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    110227-plasmid-map-sequence-id-211070

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  22. KIF3A406

    Plasmid
    #129768
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    129768-plasmid-map-sequence-id-271499

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  23. KIF3A560GFP

    Plasmid
    #129769
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129769-plasmid-map-sequence-id-263530

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  24. p2Bac.His.XPD

    Plasmid
    #39217
    Depositor
    Aziz Sancar
    Insert
    XPD (ERCC2 Human)
    Use
    Tags
    His (HHYHHH)
    Expression
    Insect
    Mutation
    XPD starts at aa4
    Promoter
    Pph
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  25. ACOT11A

    Plasmid
    #42424
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACOT11A (ACOT11 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. pBlue-hFX-HA(NcoI/EcoRI)

    Plasmid
    #15405
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    insert can be released by digestion with REs NcoI…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15405-plasmid-map-sequence-id-203076

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  27. ACOT12BA

    Plasmid
    #42395
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACOT12BA (ACOT12 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. BC-Sig-GPRC

    Plasmid
    #44201
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment with signal peptide (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    The C-terminal fragment of GPR56, starting at am…
    Promoter
    LTR
    Available since
    July 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    44201-plasmid-map-sequence-id-60060

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  31. mCherry-Sequestosome1-C-18

    Plasmid
    #55131
    Purpose
    Localization: Membrane Scaffold, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Sequestosome1 (SQSTM1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Starts at amino acid 86 of Sequestosome1
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. HOXA2 (murine) HIS-tag pET

    Plasmid
    #8557
    Depositor
    Corey Largman
    Insert
    HOXA2 (Hoxa2 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    contains 5 additional amino acids from 5' un…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  36. mVenus-Sequestosome1-C-18

    Plasmid
    #56392
    Purpose
    Localization: Membrane Scaffold, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Sequestosome1 (SQSTM1 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Starts at amino acid 86 of Sequestosome1
    Promoter
    CMV
    Available since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. pmCherry-C1-PHOSPHO1

    Plasmid
    #224896
    Purpose
    Express N-terminal mCherry fused to human PHOSPHO1 in mammalian cells
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    start codon is deleted
    Promoter
    CMV
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    224896-plasmid-map-sequence-id-456142

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  39. pMW-PahZ1 KT-1

    Plasmid
    #141134
    Purpose
    Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).
    Depositor
    Mitch Weiland
    Article
    Bolay et al Journal of Chemical Education
    Insert
    PahZ1
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Not the full-length gene. PahZ1 fragment starts a…
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141134-plasmid-map-sequence-id-308652

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Showing: 2081 - 2120 of 2761 results