We narrowed to 1,732 results for: FAST

1. pJ414-PCY1:H695A

   Plasmid

   #98155

   Purpose

   Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695A
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to alanine

   Promoter

   T7

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ414-PCY1:Y696G

   Plasmid

   #98156

   Purpose

   Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:Y696G
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed tyrosine 696 to glycine

   Promoter

   T7

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZA12

   Plasmid

   #158490

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Mutation

   L17E, D489V, no STOP codon

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC3.1_CMV_oROS-G_LF(C199S)

   Plasmid

   #216112

   Purpose

   Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-G_LF(C199S)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   pHR-CMV-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b_H6-Smt3-AesC-GFP

   Plasmid

   #234486

   Purpose

   Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

   Depositor

   Henning Mootz

   Article

   Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

   Insert

   C-terminal Fragment of the Aes123 PolB1 Split Intein
   

   Tags

   Hexahistidine Tag, Superfolder Green Fluorescent …

   Expression

   Bacterial

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL-c2X_MBP-CLmN-H6

   Plasmid

   #234487

   Purpose

   Maltose binding protein N-terminally fused to His-tagged CLmN split intein

   Depositor

   Henning Mootz

   Article

   Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

   Insert

   N-terminal Fragment of the CLm Split Intein
   

   Tags

   Hexahistidine Tag and Maltose Binding Protein (MB…

   Expression

   Bacterial

   Mutation

   T69K, F75H, M118N within the N-terminal fragment …

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

   Plasmid

   #158962

   Purpose

   Cre-dependent Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   hsp70l promoter
   floxed DsRed cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

   Plasmid

   #113885

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR-HOT-P2A-Clover-BlastR

    Plasmid

    #138568

    Purpose

    Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    P2A-clover-BlastR
    

    Use

    CRISPR

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

    Plasmid

    #113888

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRibo-Sec-APEX2m

    Plasmid

    #176844

    Purpose

    Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

    Depositor

    Jessica Seeliger

    Article

    Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

    Insert

    pRibo-Sec-APEX2m
    

    Use

    Inducible

    Expression

    Bacterial

    Promoter

    pRibo, theophilline riboswitch inducible hsp60 pr…

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR-HOT-Clover-BlastR

    Plasmid

    #138569

    Purpose

    Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    Clover-BlastR
    

    Use

    CRISPR

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-CMV-TetO2_HA-BirA-ER

    Plasmid

    #113897

    Purpose

    Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    E. coli biotin ligase
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-CMV-TetO2_HA-BirA

    Plasmid

    #113896

    Purpose

    Mammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    E. coli biotin ligase
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

    Plasmid

    #113889

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGY577

    Plasmid

    #166663

    Purpose

    Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    Cre: E340A, D341A

    Promoter

    CMV

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

    Plasmid

    #113886

    Purpose

    Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDx_mScarlet3-NCwt-SYFP2

    Plasmid

    #189766

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-NCwt-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    encodes the expected internal mutations relative …

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-CMV-TetO2_3C-mVenus-Twin-Strep

    Plasmid

    #113891

    Purpose

    Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-mVenus-Twin-Strep

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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