Addgene

Showing: 1041 - 1060 of 9629 results

Results per page:

Sort results by:

pRSETA-qmTQ2-glucose

Plasmid

#225252

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-glucose

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETA-qmTQ2-cAMP

Plasmid

#225249

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG162

Plasmid

#202581

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG163

Plasmid

#202582

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG165

Plasmid

#202584

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG160

Plasmid

#202579

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG159

Plasmid

#202578

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG1.1-Tsks/1D4

Plasmid

#199623

Purpose

Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tsks (variant X6) (Tsks Mouse)

Use

Tags

1D4

Expression

Mutation

Promoter

CAG

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG1.1-HA/Ppp1cc2

Plasmid

#199626

Purpose

Expression vector of mouse PPP1CC2. HA tag at N-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Ppp1cc mRNA (variant X1) (Ppp1cc Mouse)

Use

Tags

HA-tag

Expression

Mutation

Promoter

CAG

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Use

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pElectra1-LaminA

Plasmid

#179482

Purpose

Nuclear envelope labeling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-LaminA

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-TOMM20-Halo

Plasmid

#129348

Purpose

Halo Tag, Mitochondrial outer membrane localization

Depositor

Yasushi Okada

Article

Wang et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

Halo (Promega)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MPC021: CamKII CMV_sNovArch_citrine (soma targeting)

Plasmid

#153194

Purpose

encoded soma targeted NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

sNovArch

Use

Tags

citrine

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

Plasmid

#124181

Purpose

piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

IRES-Pac, Pou5f1 enhancer

Use

Reporter

Tags

Expression

Mutation

Promoter

UBCmin

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2

Plasmid

#124175

Purpose

piggyBac-based Dox-inducible expression (very weak)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

Plasmid

#124180

Purpose

piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

EGFP-IRES-Pac, Pou5f1 enhancer

Use

Reporter

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YORWΔ22Ω2

Plasmid

#101705

Purpose

GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
YORWΔ22Ω1

Plasmid

#101706

Purpose

GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
YORWΔ22α2

Plasmid

#101707

Purpose

GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only
YORWΔ22α1

Plasmid

#101708

Purpose

GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge