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pcDNA4/TO-Flag-H3.3

Plasmid

#47980

Purpose

tetracycline-inducible expression of human Flag tagged H3F3A

Depositor

Bing Zhu

Article

Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

Insert

H3F3A (H3-3A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

288 bp T to C synonymous mutation

Promoter

CMV promoter with tetracycline operator

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pLL3.7-CAG-hMGT-Ef1a-puro

Plasmid

#172395

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7-UBCp-hMGT-Ef1a-puro

Plasmid

#172396

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-human LRRC33

Plasmid

#111602

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Use

Tags

myc/his tag

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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PCBP1_Full-length_pGEX

Plasmid

#135108

Purpose

Recombinant expression of PCBP1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PCBP1 (PCBP1 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAX-mScarlet-FBP17

Plasmid

#178366

Purpose

Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

400 Glutamic Acid changed to Aspartic acid (403 A…

Promoter

Chicken Beta Actin

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-PHD2

Plasmid

#223552

Purpose

Lentivirus transfer plasmid for expression of full length human PHD2

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSG5-hEKLF

Plasmid

#67835

Purpose

Expression of human EKLF driven by SV40 promoter for use in activation studies

Depositor

James Bieker

Article

Mas et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

EKLF (KLF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.18 NR0B1-Luc

Plasmid

#100983

Purpose

NR0B1 luciferase reporter - firefly luciferase driven by NR0B1 promoter

Depositor

Lee Helman

Article

Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

Insert

NR0B1 (NR0B1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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IGF2BP1_2xRRM-4xKH_pGEX

Plasmid

#135122

Purpose

Recombinant expression of IGF2BP1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

IGF2BP1 (IGF2BP1 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

Plasmid

#199197

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int-BlastR

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

Plasmid

#199198

Purpose

Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int-G418R

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMG046 pETARA-CK1α-His

Plasmid

#154074

Purpose

Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Tags

GST and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-mApple

Plasmid

#193780

Purpose

Tricistronic expression of Cas9-mApple-BSD in mammalian cells

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

mApple

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

In frame with Cas9

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199195

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199196

Purpose

Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTMB341_ZFcharm_Prnp_SCR

Plasmid

#220844

Purpose

AAV genome expressing ZFcharm targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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HA-DN-14-3-3 zeta (R56A/R60A)

Plasmid

#116889

Purpose

Expresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cells

Depositor

Ken-Ichi Takemaru, FENG-QIAN Li

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

DN-14-3-3 zeta (R56A/R60A) (YWHAZ Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEF019 MBP-β-catenin-His

Plasmid

#154070

Purpose

Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CTNNB1 (CTNNB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sPDGFRalpha-Fc

Plasmid

#136438

Purpose

Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Use

Tags

Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

Expression

Mammalian

Mutation

Extracellular domain of PDGFR-alpha (a.a. 1-524)

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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