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pCAG-hGPR56-IRES-GFP

Plasmid

#52297

Purpose

Expresses human GPR56 and GFP in mammalian cells.

Depositor

Christopher A Walsh

Publication

Bae et al Science. 2014 Feb 14;343(6172):764-8. do

Insert

Human GPR56 coding sequence (ADGRG1 Human)

Expression

Mammalian

Promoter

CAG promoter

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGDP2 MCR-1

Plasmid

#118404

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.

Depositor

Gerard Wright

Publication

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

MCR-1

Expression

Bacterial

Promoter

pLac

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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6920 pPROEXHsp104b

Plasmid

#1230

Depositor

Susan Lindquist

Publication

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (HSP104 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBabe puro RalA Q75L

Plasmid

#19719

Depositor

Channing Der

Publication

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

GTPase deficient & constitutively active Gl…

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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OPA1-isoform1

Plasmid

#70173

Purpose

Mammalian expression of hsOPA1 isoform 1

Depositor

David Chan

Publication

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 1 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLX301-ZIM3-KRAB-PYL1

Plasmid

#154761

Purpose

Expresses ZIM3 KRAB domain fused to PYL1

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

CMV

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Publication

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Mutation

D10A

Promoter

j23119 and ptb

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Publication

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Expression

Bacterial

Promoter

araBAD

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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2Flag-pDEST-N

Plasmid

#118371

Purpose

Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCdrA-gfpC

Plasmid

#111614

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Publication

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pKM631

Plasmid

#17727

Depositor

David Waugh

Publication

Kapust et al Biochem Biophys Res Commun. 2002 Jun 28.

Insert

NusG (nusG Aquifex aeolicus)

Tags

His6 and MBP

Expression

Bacterial

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV_psychLight2

Plasmid

#163910

Purpose

Expresses psychLight2 in mammalian cells

Depositor

Lin Tian

Publication

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Insert

psychLight2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMpGE_En03

Plasmid

#71535

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi, Sumihare Noji

Publication

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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FlucDM-HA-GFP11-N1

Plasmid

#91956

Purpose

Expresses aggregation-prone FlucDM-HA-GFP11 in mammalian cells

Depositor

Rong Li

Publication

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucDM-HA-GFP11

Expression

Mammalian

Mutation

R188Q,R261Q

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAXY0001

Plasmid

#179834

Purpose

Binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Publication

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Insert

eYGFPuv

Use

Binary vector for plant transformation

Expression

Plant

Promoter

35S

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCHERRY10

Plasmid

#24664

Depositor

Tanya Parish

Publication

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pIM1773-PRHIII-sfGFP-pNW33N

Plasmid

#52217

Purpose

Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli

Depositor

Ichiro Matsumura

Publication

Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Insert

PRHIII-sfGFP

Expression

Bacterial

Promoter

PRHIII

Available since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox1

Plasmid

#58344

Purpose

expresses human Nox1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Publication

Banfi et al Science. 2000 Jan 7;287(5450):138-42.

Insert

NADPH oxidase 1 (NOX1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSKDuet16

Plasmid

#41684

Depositor

Hideo Iwai

Publication

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

NpuDnaE intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pNPTS138 Cm

Plasmid

#41891

Depositor

Howard Steinman

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb

Expression

Bacterial

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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