We narrowed to 14,753 results for: RING

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pMV-Tc1-2_ST

Plasmid

#217289

Purpose

Donor plasmid of Tc1-2_ST Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tc1-2_ST transposon

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-piggyBac-1_AMi

Plasmid

#217236

Purpose

Donor plasmid of piggyBac-1_AMi Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of piggyBac-1_AMi transposon

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet2 promoter F

Plasmid

#63879

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 Promoter fragment F

Use

Luciferase

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP28

Plasmid

#41673

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 28 C-terminal-…

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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VanGlow-RR-DSCP

Plasmid

#83339

Purpose

for Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

Depositor

Cheng-Yu Lee

Article

Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

Type

Empty backbone

Use

Luciferase

Tags

mCherry::Renilla(nls)

Expression

Insect

Promoter

Drosophila Synthetic Core Promoter

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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J-sRNA-331Bb

Plasmid

#78285

Purpose

An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

j-sRNA-331

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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dCas9-DNMT3L

Plasmid

#126588

Purpose

DNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

DNMT3L (DNMT3L Human)

Use

CRISPR

Tags

3XFLag-NLS-dCas9-DNMT3L-NLS

Expression

Mammalian

Available Since

Dec. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2021

Plasmid

#49128

Purpose

AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple302-iCre

Use

AAV

Expression

Mammalian

Promoter

DCX

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-shSmad2

Plasmid

#37051

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad2 (SMAD2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
SuExp His-Myc-mPLD4

Plasmid

#201185

Purpose

Protein expression plasmid for recombinant His-Myc tag mouse PLD4

Depositor

David Nemazee

Article

Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

Insert

PLD4 (Pld4, Pld4, Pld4 Mouse)

Tags

His-Myc, Factor Xa cleavable

Expression

Mammalian

Mutation

cytosolic and transmembrane domain truncated; add…

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJZC48

Plasmid

#62336

Purpose

sgRNA + 1x COM with COM-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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FpG5.BFP.Reporter

Plasmid

#134967

Purpose

Lentiviral vector for enhancer reporter assay

Depositor

Beat Bornhauser

Article

Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

mini Fgf4 promoter

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130993

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC620

Plasmid

#62282

Purpose

Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
PCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Nuclease activity has been inactivated by mutatio…

Promoter

pAdh and pTdh3

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-Gluc-RMA-IRES-EGFP

Plasmid

#189628

Purpose

Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

Gaussia luciferase fused to Fc
IRES-EGFP

Use

AAV and Luciferase

Tags

Gluc and IgG1 Fc

Expression

Mammalian

Promoter

IRES and TRE

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTriex-mVenus-PI-RhoA(Q63L)

Plasmid

#86978

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
RhoA (RHOA Human)

Tags

6xHis and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-E6AP Hect

Plasmid

#165102

Purpose

bacterial expression of a GST fusion of Human E6AP Hect domain

Depositor

Arthur Haas

Article

Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

Insert

E6AP Hect (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

E6AP Hect domain Amino acids 495-738 (5'QLNP…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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Ana GvpC-SpyTag-His in pET28a

Plasmid

#85737

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (fused to SpyTag and His-tagged)

Tags

His-tag and SpyTag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

Plasmid

#99229

Purpose

Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos2

Tags

Lifeact

Expression

Mammalian

Mutation

A69T for primed conversion applications

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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