165,725 results

1. pCDNA3.1(+)-NEMOm

   Plasmid

   #189930

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOs
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD373

   Plasmid

   #137021

   Purpose

   SapTrap GFP-GLO donor

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   C. elegans GLO-optimized GFP
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized GFP

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-CAG-hrGFP

   Plasmid

   #52344

   Purpose

   AAVS1 donor vector for constitutive gene expression

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   hrGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS714

   Plasmid

   #188886

   Purpose

   MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Pmex-5::Cas9
   

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL025_HA-rb_IgG_HC_15F11

   Plasmid

   #193622

   Purpose

   HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti_HA_15F11 rabbit heavy chain
   

   Tags

   signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDL026_HA-rb_IgG_LC_15F11

   Plasmid

   #193623

   Purpose

   HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-HA_15F11 rabbit light chain
   

   Tags

   signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. HA-VHL-pRc/CMV

   Plasmid

   #19999

   Depositor

   William Kaelin

   Article

   Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wild type

   Available Since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-HA-USP30

   Plasmid

   #22578

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP30 (USP30 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVH-EF1a-GFP-P2A-MYC

   Plasmid

   #130695

   Purpose

   Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human MYC (MYC Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. moxCerulean3

    Plasmid

    #68066

    Purpose

    C-terminal fusion to moxCerulean3

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Tags

    moxCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-KIR2DL4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156662

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL4 (KIR2DL4 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ancBE4-SpRY

    Plasmid

    #226855

    Purpose

    Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ancBE4max-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158990

    Purpose

    Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. dg264_pENTR_ChronosCel

    Plasmid

    #66102

    Purpose

    Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chronos
    

    Use

    Gateway

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid

    #160437

    Purpose

    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
    

    Tags

    Cleavage signal

    Expression

    Bacterial

    Mutation

    Codon-optimized for expression in C. elegans

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174529

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Inserts

    PylS
    PylT
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p221a-GUS

    Plasmid

    #71263

    Purpose

    Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Beta-glucuronidase
    

    Use

    Gateway

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6/V5-DEST-SPAG5

    Plasmid

    #31217

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    SPAG5 (SPAG5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Contains a one nucleotide deletion at C-term that…

    Available Since

    Aug. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pFastBac1-6His-DHX9

    Plasmid

    #211440

    Purpose

    generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

    Depositor

    Frank Grosse, Helmut Pospiech

    Article

    Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

    Insert

    DHX9 (DHX9 Human)
    

    Tags

    6xHis

    Expression

    Insect

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNCS mStrawberry

    Plasmid

    #91768

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Strawberry
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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