We narrowed to 12,202 results for: 112

1. CHRM4-DuET

   Plasmid

   #213210

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CHRM4 (CHRM4 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FusionRed-CD36-C-10

   Plasmid

   #56104

   Purpose

   Localization: Membrane, Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Insert

   CD36 (CD36 Human)
   

   Tags

   FusionRed

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pAAV-hSyn-DIO-GRAB_CCK1.0

   Plasmid

   #208674

   Purpose

   Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac(His10-Shp2)

   Plasmid

   #177899

   Purpose

   Baculo expression of His10 tag fused to human Shp2

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   Shp2 (PTPN11 Human)
   

   Tags

   10xHis-TEV-SNAP tag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-GRAB_NPYmut

   Plasmid

   #208679

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSR:MSMEG_5048:Dendra

   Plasmid

   #167366

   Purpose

   C-term fluorescent fusion

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_5048
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Promoter

   pmycTetO

   Available Since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur

   Plasmid

   #69052

   Purpose

   lentiviral expression of IKZF2 variant 2 with HA tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF2 (IKZF2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   splice variant 2

   Promoter

   UBC

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307-HCoV-NL63-3CL-C144A

   Plasmid

   #160287

   Purpose

   Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C144A

   Promoter

   EF1a

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX307-SARS-CoV-3CL-C145A

   Plasmid

   #160281

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

    Plasmid

    #108484

    Purpose

    inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CenpB-dCEN-INCENP (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CEN box replaced by CenpB 1-166

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA6-N-3XFLAG-Fzd6

    Plasmid

    #123588

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    frizzled class receptor 6 (Fzd6 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSICO-CPSF6-iRFP670

    Plasmid

    #167586

    Purpose

    Expresses human CPSF6 fused to iRFP

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    CPSF6-iRFP670 (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Mutation

    WT

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSR:MSMEG_0028:Dendra

    Plasmid

    #159038

    Purpose

    T7 expression of C-term fluorescent fusion in E. coli

    Depositor

    Joseph Wade, Keith Derbyshire

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_0028
    

    Use

    Integrating mycobacterial expression vector attl5

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Promoter

    pmycTetO

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-tetO-GFI1

    Plasmid

    #139813

    Purpose

    doxycycline-inducible expression of Human GFI1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    GFI1 (GFI1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    P2L- Please see depositor comment below

    Promoter

    TRE-mCMV

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRevTRE MKL1-N100 S449A/T450A/S454A

    Plasmid

    #19849

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1-N100 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    deleted amino acids 1-100 and changed Serine 449,…

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pGL3-SV40P-Tet2 enhancer H1

    Plasmid

    #63883

    Purpose

    Luciferase reporter plasmid used to study transcriptional control of murine Tet2

    Depositor

    Kian Peng Koh

    Article

    Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

    Insert

    Tet2 enhancer fragment H1
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

    Plasmid

    #176535

    Purpose

    Non-protein coding locus components from CRISPR-associated IscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-hPMCA2xb

    Plasmid

    #47751

    Purpose

    mammalian expression of mCherry tagged hPMCA2, xb splice variant

    Depositor

    Emanuel Strehler

    Article

    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    xb splice variant

    Promoter

    CMV

    Available Since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pDisplay-GRAB_CRF0.5

    Plasmid

    #208654

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX307-MERS-CoV-3CL-C148A

    Plasmid

    #160283

    Purpose

    Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C148A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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