We narrowed to 14,795 results for: flag
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Plasmid#179448PurposeDonor vector to knock in mClover3 N-terminal to human PER2 geneDepositorInsertmClover3
UseCRISPRTagsHis/FlagPromoterNoneAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
4LNDDE488Q-pCDNA3.1(+)
Plasmid#172228PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
Tags6xHis and FlagExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Spike Display_B.1.427/B.1.429
Plasmid#172738PurposeExpression vector for SARS-CoV-2 (B.1.427/B.1.429) spike used in the Spike Display systemDepositorInsertSARS-CoV-2 S B.1.427/B.1.429 (S Severe acute respiratory syndrome coronavirus 2)
Tags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterCMVAvailable SinceAug. 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
Plasmid#163698PurposeAAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortexDepositorInsertCaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMammalianPromoterCaMKII fragmentAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB701NL3F10H
Plasmid#141288PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)DepositorTypeEmpty backboneUseGateway Cloning and LuciferaseTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-mPD-L1 3SA
Plasmid#121489PurposeExpression of mouse PD-L1 3SADepositorInsertPD-L1 3SA
UseLentiviralTagsFlagExpressionMammalianMutationS175A, T179A, S183APromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SaLgCP
Plasmid#164562PurposePuroDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsFlag-NLS-SaCas9-P2A-PuroRExpressionMammalianPromoterU6 promoter for crRNA expression and EFS promoter…Available SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75S)
Plasmid#137828PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75S)
Tags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Serine in NTEVp fragmentPromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
K20‐scFv‐pSMBP2
Plasmid#159940PurposeTo generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20DepositorInsertanti beta-1 integrin scFv
Tags6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…ExpressionInsectPromoterPolyhedrinAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG645_lexO-AscI_LexA-TF_LEU2MX
Plasmid#154813PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7pr-His6-MBP-TEV-dCas9-BirA*
Plasmid#159994PurposeBacterial expression of dCas9-BirA*DepositorInsertdCas9-BirA*
Tags6X-His, MBP, 3X-FLAG, NLSExpressionBacterialMutationD10A, H840APromoterT7Available SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME18S-F-R-Olfr160
Plasmid#149366PurposeExpresses Olfr160 in mammalian cells.DepositorAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep131
Plasmid#136875PurposeMammalian expression of the centrosomal protein Cep131 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep131 (CEP131 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Centrin3
Plasmid#136806PurposeMammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin3
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep78
Plasmid#136827PurposeMammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep78 (CEP78 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep72
Plasmid#136819PurposeMammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep72 (CEP72 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep57
Plasmid#136817PurposeMammalian expression of the centrosomal protein Cep57 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep57 (CEP57 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-STIL
Plasmid#136795PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tagDepositorInsertSNAP-STIL (STIL Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-POC5
Plasmid#136786PurposeMammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tagDepositorInsertSNAP-POC5 (POC5 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry
Plasmid#135014PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
Tags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the C-termi…PromoterCBhAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only