We narrowed to 17,163 results for: EMB

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NES-PAmCherry-cPHx3

Plasmid

#116861

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

PAmCherry

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Igfbp1-promoter/pGL3

Plasmid

#12146

Depositor

Domenico Accili

Article

Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

Insert

IGFBP1 promoter (Igfbp1 Rat)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains the canonical insulin response sequence …

Available Since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-cGAS R71/75E-HA

Plasmid

#130919

Purpose

Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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p3s-ABE7.10

Plasmid

#113128

Purpose

expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE 7.10

Tags

HA epitope and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pINT-PuroDDHNF4a2

Plasmid

#31443

Depositor

Gerhart Ryffel

Article

Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

Insert

HNF4A (HNF4A Human)

Use

Phic31/attb

Tags

FKBP L106P

Expression

Mammalian

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pT5T_hp53_ST

Plasmid

#34922

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 1-393) (TP53 Human)

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 DLC1

Plasmid

#24266

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1, DLC1 Human)

Tags

T7

Expression

Mammalian

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
p3E-V5-mScarlet-I_SV40pA (JDW 968)

Plasmid

#224529

Purpose

A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII

Plasmid

#217896

Purpose

Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMB5 (PSMB5 Human)
PSMB6 (PSMB6 Human)
PSMB7 (PSMB7 Human)

Tags

2xSTII

Expression

Insect

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA5-PSMA6-PSMA7

Plasmid

#217894

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPc

Plasmid

#87166

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

YFPc

Promoter

35s

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TRAIL-R4

Plasmid

#61385

Purpose

Expresses HA tagged TRAIL receptor 4

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 4 (TNFRSF10D Human)

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R4 leader peptide (aa1-29) replaced with TN…

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pFL His-mEos3.2-I3

Plasmid

#113516

Purpose

Expression of His-Eos-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Tags

6*His-mEos3.2

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMMDS DEST

Plasmid

#206264

Purpose

DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Type

Empty backbone

Use

Cre-donor, multimate/gateway dest vector (multiba…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPn

Plasmid

#87165

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

YFPn

Promoter

35s

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_M_HA_BASU

Plasmid

#153990

Purpose

Express M gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKY925F

Plasmid

#216547

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: Y925F ) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (Y925F) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation Y925F

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-NLS-Beta-catenin-S33Y

Plasmid

#138537

Purpose

For making Beta-catenin-S33Y RNA

Depositor

Carmen Williams

Article

Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

Insert

Beta-catenin

Use

In vitro transcription

Tags

2XFLAG and 2xNLS

Mutation

changed serine 33 to tyrosine

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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