164,826 results

1. pRGEN-CMV-CjCas9

   Plasmid

   #89752

   Purpose

   Expression of CjCas9 in mammalian cells

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pet-MBP

   Plasmid

   #209184

   Purpose

   Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Type

   Empty backbone

   Use

   Tags

   HisX6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTriEx4-H6-FGAm (FlincG3)

   Plasmid

   #49202

   Purpose

   Fluorescent reporter for cGMP

   Depositor

   John Garthwaite

   Article

   Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

   Insert

   FlincG3
   

   Use

   Tags

   His tag + other tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Met to Lys in cpEGFP region similar to CGaMP3

   Promoter

   CMV

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.hSynap.ArcLightD.WPRE.SV40

   Plasmid

   #100037

   Purpose

   AAV expression of ArcLightD driven by hSynap promoter

   Depositor

   Vincent Pieribone

   Insert

   ArcLightD
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynap

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pErbB2-ECFP

   Plasmid

   #66945

   Purpose

   Mammalian expression of rat ErbB2 tagged with ECFP

   Depositor

   Bo van_Deurs, Mads Lerdrup

   Article

   Lerdrup et al J Cell Sci. 2006 Jan 1;119(Pt 1):85-95.

   Insert

   ErbB2 (Erbb2 Rat)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pAV-U6+27-Tornado-RhoBAST-MS2

   Plasmid

   #196342

   Purpose

   Overexpression of circular RhoBAST-MS2

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   Tornado-RhoBAST-MS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Reb_206 aka PAS629

   Plasmid

   #71234

   Purpose

   Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   mNeonGreen to second copy RebB, soluble mCherry a…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR1A no ccDB (w48-1)

   Plasmid

   #17398

   Purpose

   Gateway entry 1A vector, no ccDB gene

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-SYN-mBaoJin-oPRE

   Plasmid

   #218683

   Purpose

   AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

   Depositor

   Bart Nieuwenhuis

   Insert

   mBaoJin
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human synapsin promoter

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SPDK3876(TRV-RNA2-pPEBV-MCS)

    Plasmid

    #149275

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Promoter

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-EPG

    Plasmid

    #117978

    Purpose

    Express EPG in mamalian cells

    Depositor

    Assaf Gilad

    Article

    Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1

    Insert

    Electromagnetic perceptive gene
    

    Use

    Tags

    HIS and v5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSUMO1

    Plasmid

    #52258

    Purpose

    Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-1 (SUMO1 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 )
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PresentER-SIINFEKL (mCherry)

    Plasmid

    #102945

    Purpose

    PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    SIINFEKL (OVAL Synthetic, Chicken)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    OVA257-264

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pRRL Luciferase

    Plasmid

    #120798

    Purpose

    For production of lentivirus to express luciferase, primarily for in vivo bioluminescence tracking experiments.

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Puro-iNgn3

    Plasmid

    #75337

    Purpose

    doxycycline inducible Ngn3 over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    neurogenin 3 (Neurog3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMi(3xP3-DsRed)

    Plasmid

    #102539

    Purpose

    Transgenesis vector based on the Minos transposon, carrying the 3xP3-DsRed marker

    Depositor

    Michalis Averof

    Article

    Pavlopoulos et al Proc Natl Acad Sci U S A. 2005 May 31;10

    Insert

    Minos transpoable element with 3xP3-DsRed marker
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNLS-iRFP713

    Plasmid

    #45468

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP713
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIRE YIC

    Plasmid

    #18784

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Insert

    H-ferritin iron response element
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid

    #122846

    Purpose

    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    human FASN/ human fatty acid synthase (FASN Human)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge