We narrowed to 19,314 results for: Tat
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Plasmid#77871Purpose3rd generation lentiviral gRNA plasmid targeting human TAF1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only
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pTT5-hSDC1-S15A
Plasmid#52363Purposeexpresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
ExpressionMammalianMutationS15A and R95Q (according to numbering in Fig. 2A …PromoterCMVAvailable SinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-MIII
Plasmid#58683PurposeExpresses LGP2 with a mutation to motif III in mammalian cellsDepositorInsertLGP2-MIII (DHX58 Human)
Tags3x FLAGExpressionMammalianMutationthreonine and serine of helicase motif III mutate…PromoterCMVAvailable SinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
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K15/pGL3
Plasmid#44264DepositorInsertK15 promoter (-4.8) (Krt15 Mouse)
UseLuciferase and Mouse TargetingTagsLuciferaseExpressionMammalianMutationContains the murine K15 promoter (-4.8) fragmentPromotermurine K15 promoter (-4.8) fragmentAvailable SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat EE
Plasmid#42569DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins
Plasmid#31440DepositorAvailable SinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH399-SUP45-S30P
Plasmid#29372DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationS30P mutant gene (corresponding to the sl23ts all…Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
EC.SurA.pTYB1.a21-281&390-428.L239R
Plasmid#15959DepositorInsertEscherichia coli surA delta PPIase domain 2
TagsinteinExpressionBacterialMutationresidues 282-389 deleted; L239R mutationAvailable SinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28(a)-hFX(I154F)-HA
Plasmid#14992DepositorInserthuman frataxin cDNA (FXN Human)
TagsAU1, HA, and HISExpressionBacterialMutationcontains cDNA coding sequence for amino acids 88-…Available SinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX(I154F)(AA88)-HA
Plasmid#15403DepositorInserthuman frataxin cDNA (FXN Human)
TagsAU1, HA, and HISExpressionBacterialMutationcontains cDNA coding sequence for amino acids 88-…Available SinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
B8LUI6
Plasmid#163251PurposeInducible expression of UniProt identifier B8LUI6DepositorInsertB8LUI6
Tags10xHis and T7ExpressionBacterialPromoterT7Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
B8MMC0
Plasmid#163270PurposeInducible expression of UniProt identifier B8MMC0DepositorInsertB8MMC0
Tags10xHis and T7ExpressionBacterialPromoterT7Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
C9Y9E7
Plasmid#163230PurposeInducible expression of UniProt identifier C9Y9E7DepositorInsertC9Y9E7
Tags10xHis and T7ExpressionBacterialPromoterT7Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY9
Plasmid#130907PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-2G6, and PtetAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME270
Plasmid#238067PurposeExpresses ME-ABE ABE7.10-HRHSK with SpCas9 (NG)DepositorInsertTadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpnCas9(D10A)
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationTadA modifications include L36H, L51R, Y123H, C14…Available SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGASdeltaN R255E-HA
Plasmid#186894PurposeDoxycycline-dependent expression of human cGAS gene (with R255E mutation) in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with R255E mutation and C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsHAMutationN-terminal truncation, R255EPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast 3xFlag-SPNS1(E164K)
Plasmid#198569PurposeSPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vectorDepositorInsertSPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
UseRetroviralTags3xFlagExpressionMammalianMutationchanged glutamic acid E164 to lysine (E164K)Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-mPF-BACH1 Cas9-Resistant_AmpR
Plasmid#199221PurposeLPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInsertrtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
TagsEGFPExpressionMammalianMutationsilient mutation at nucleic acid 177 C changed to…Promotertight TRE promoterAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only