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We narrowed to 16,183 results for: nes

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Showing: 10661 - 10680 of 16183 results

  1. pAAV-hsyn-DIO-AppNL-G-F-T2A-mCherry

    Plasmid
    #242649
    Purpose
    Floxed (DIO) AAV transgene designed to be packaged in AAV and to express the amyloid precursor protein (App) with familial mutations AppNL-G-F in a Cre-recombinase-dependent manner.
    Depositor
    William Wisden
    Insert
    Floxed (DOI) AAV transgene expressing an amyloid precursor protein with familial mutations and an mCherry tag
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Mutation
    The amyloid precursor reading frame contains a c…
    Promoter
    human synapsin
    Available since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    242649-plasmid-map-sequence-id-489352

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  3. pT2iU6m5Hoxa13bE4PCh(#765)

    Plasmid
    #184102
    Purpose
    cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-ZfHoxa13b-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes) + in vitro tra…
    Tags
    5xMyc
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Ubi+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184102-plasmid-map-sequence-id-365950

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  4. pT22cUm5En2E4P3Che(#1188)

    Plasmid
    #184094
    Purpose
    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Mutation
    5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184094-plasmid-map-sequence-id-363967

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  6. Epac-S-H150

    Plasmid
    #170345
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170345-plasmid-map-sequence-id-344646

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  7. Epac-S-H94

    Plasmid
    #170341
    Purpose
    GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170341-plasmid-map-sequence-id-335921

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  8. pKIF1C-2xFLAG

    Plasmid
    #130979
    Purpose
    Expression of KIF1C-2xFLAG in mammalian cells.
    Depositor
    Anne Straube
    Publication
    Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF1C-2xFLAG (KIF1C Human)
    Tags
    2xFLAG
    Expression
    Mammalian
    Mutation
    4 silent mutations that make this construct resis…
    Promoter
    CMV
    Available since
    Oct. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    130979-plasmid-map-sequence-id-259357

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  9. pVZ_PcoaT_SQE1_LUP1

    Plasmid
    #103857
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Publication
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    lupeol synthase 1 (LUP1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103857-plasmid-map-sequence-id-217123

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  10. GFP-KIF1B beta-600-1400

    Plasmid
    #79109
    Purpose
    Mammalian expression of GFP-KIF1B beta-600-1400
    Depositor
    Susanne Schlisio
    Publication
    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    exon 25 missing (natural splice varient)
    Promoter
    CMV
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid
    #192443
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192443-plasmid-map-sequence-id-379076

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  14. GP804

    Plasmid
    #164548
    Purpose
    Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R
    Depositor
    Gregory Pazour
    Publication
    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Insert
    Smoothened K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    All sixteen Lysines in cytoplasmic C-terminal tai…
    Promoter
    GgCryD1
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164548-plasmid-map-sequence-id-325344

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  15. pcDNA3-mCherry-TSC2(AA,3Q)

    Plasmid
    #192444
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192444-plasmid-map-sequence-id-379131

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  16. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187949
    Purpose
    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187949-plasmid-map-sequence-id-373500

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Showing: 10661 - 10680 of 16183 results