We narrowed to 28,451 results for: tat

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TWF1 gRNA (BRDN0001149022)

Plasmid

#77287

Purpose

3rd generation lentiviral gRNA plasmid targeting human TWF1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TWF1 (TWF1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-CHD5(Chromos)-FRAG

Plasmid

#68875

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

only the two chromodomains of CHD5

Promoter

tac

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pRS416 ATG5-E141D-HA-ATG12-ATG16

Plasmid

#166851

Purpose

Expresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.

Depositor

Daniel Klionsky

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

ATG5 ATG12 ATG16 (ATG5, ATG12, ATG16 Budding Yeast)

Tags

HA-ATG12

Expression

Yeast

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-9465

Plasmid

#115464

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-9465 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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DYRK1B gRNA (BRDN0001146749)

Plasmid

#76622

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMY-Ly6G-P2A-PuroR-HA-Rluc_miR

Plasmid

#163340

Purpose

Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

Ly-6G (Ly6g Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo-TGmiR-Gb2

Plasmid

#25746

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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Chicken ArcLight S174

Plasmid

#53567

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-S174

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-H79Q

Plasmid

#98953

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant H79Q

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

H79Q: enhanced CXCL12 binding and signaling

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-GRM3 (S610L)

Plasmid

#31800

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed serine 610 to leucine

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-GRM3 (E870K)

Plasmid

#31802

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed glutamic acid 870 to lysine

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV TIP60 390-513

Plasmid

#78797

Purpose

To overexpress TIP60 390-513 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

aa390-513

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pAAV2-TBG-FLAG-mRIPK1-S321E

Plasmid

#115343

Purpose

Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321E

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Use

AAV

Tags

Flag

Expression

Mammalian

Mutation

S321E

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-T,RbsR-T]

Plasmid

#60773

Purpose

Contains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-T
GalS-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with GalS LB…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Socs2-SH2

Plasmid

#46505

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs2 (SOCS2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 28-162

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ239-RVR

Plasmid

#108863

Purpose

FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R, K629R and N633R, Cpf1 is rice codon optimi…

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G466V

Plasmid

#131722

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G466V

Promoter

CMV

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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LADL Bridge + Promoter Only Target

Plasmid

#127669

Purpose

Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

CRY2-HA-2A-mCherry and gRNAs 115 and 117

Use

CRISPR

Tags

HA

Expression

Mammalian

Promoter

EF1a and hU6

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJJR50

Plasmid

#75026

Purpose

U6 promoter driven flipped + extended sgRNA expression vector

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

guide RNA, flipped and extended version

Use

CRISPR

Promoter

R07E5.16 (U6)

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF1-GRM3 (E767K)

Plasmid

#31801

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed glutamic acid 767 to lysine

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only