We narrowed to 17,264 results for: tes

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pCDNA3_nmPKA-Cα-mPAGFP

Plasmid

#114131

Purpose

Mammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Publication

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with G2A mutation (Prkaca Mouse)

Expression

Mammalian

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMX241

Plasmid

#23017

Depositor

Linda Wordeman

Publication

Wordeman et al J Cell Biol. 2007 Dec 3. 179(5):869-79.

Insert

GCPBMhyp

Tags

CenpB 1-167 and EGFP

Expression

Mammalian

Mutation

deleted MCAK amino acids 1-178, 584-718 mutated …

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9-GFP S122D

Plasmid

#114667

Purpose

Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Publication

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

changed serine 122 to aspartic acid

Promoter

ATG9

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Publication

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant

Plasmid

#106112

Purpose

Inducible gene expression of FlagHA-PRKRA sgRNA PAM mutant

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

silent mutation of Gly32 (GGG-->GGT)

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_CELF1

Plasmid

#106101

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF1

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting CELF1 (CELF1 Human)

Use

CRISPR

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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GST-AR-4-52

Plasmid

#104199

Purpose

Expresses human AR-4-52 in in pGEX-4T-1 as GST fusion protein

Depositor

Elizabeth Wilson

Publication

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

human androgen receptor aa 4-52 (AR Human)

Tags

GST

Expression

Bacterial

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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AtFLS2pro::GUS

Plasmid

#87167

Purpose

expression pattern of AtFLS2

Depositor

Silke Robatzek

Publication

Beck et al J Exp Bot. 2014 Dec;65(22):6487-98. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AT5G46330 promoter

Tags

GUS

Promoter

AtFLS2

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p5A7-T

Plasmid

#92020

Purpose

Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease

Depositor

Brian Pfleger

Publication

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Inserts

TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
Tobacco Etch Virus Protease

Use

TALEN

Tags

5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

Mutation

S219V

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP

Plasmid

#89062

Purpose

AAV-gRNA targeting the murine Apob gene

Depositor

William Lagor

Publication

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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CIP2Aprom27bp-pGL4.10Luc

Plasmid

#60876

Purpose

Luc reporter driven by 27 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Publication

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

27 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Available since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
pTAtUbq3 (GB0219)

Plasmid

#68192

Purpose

Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq3 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPAct2noUTR (GB0192)

Plasmid

#68177

Purpose

Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 promoter (no UTR)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pWPXL-resNP1

Plasmid

#65061

Purpose

Constitutive expression of NP1 resistant to silencing by RNAi with pLVTH-shNP1

Depositor

Ramon Trullas

Publication

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

Neuronal Pentraxin 1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

G447A, C450T, A451T, G452C, C454A, C456A, C459G

Promoter

EF1alpha

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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Luc-Hdm4-3'UTR-F2d5

Plasmid

#64018

Purpose

Luciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5

Depositor

Lin He

Publication

Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

Insert

HDMX (MDM4 Human)

Expression

Mammalian

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_38

Plasmid

#60279

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Publication

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ACSL1 enhancer (ACSL1 Human)

Use

Gateway Cloning

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pIhh715bp-Luc

Plasmid

#53606

Purpose

Reporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

Depositor

Xiangli Yang

Publication

Wang et al Development. 2009 Dec;136(24):4143-53. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ihh promoter

Use

Luciferase; Reporter

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only
p413TEF-CARKL

Plasmid

#51747

Purpose

overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

Depositor

Markus Ralser

Publication

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Expression

Yeast

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-PYD-GST

Plasmid

#51531

Purpose

Express GST-tagged PYD of hAIM2 in bacteria

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GST and T7

Expression

Bacterial

Mutation

deletion of amino acids 102-343

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-09 FL C214S

Plasmid

#47086

Depositor

Marina Ramirez-Alvarado

Publication

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

C214S

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only