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pHAGE-AKAP13__RET

Plasmid

#116806

Purpose

Lentiviral expression of AKAP13__RET fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKAP13__RET

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 DEPTOR

Plasmid

#21337

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR shRNA 1 (Deptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 7, 2009

Availability

Academic Institutions and Nonprofits only
pHAGE-EGFR__ERP44

Plasmid

#116815

Purpose

Lentiviral expression of EGFR__ERP44 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR__ERP44

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-sTagRFP-TUBA1B

Plasmid

#207769

Purpose

Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBpuro - HA KLF4 FL

Plasmid

#34589

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-Atoh1

Plasmid

#158529

Purpose

Expresses mouse Atoh1 in mammalian cells

Depositor

Neil Segil

Article

Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

Insert

Atonal BHLH Transcription Factor 1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ROBO4-COMP5AP-AviTag-9xHis

Plasmid

#157428

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ROBO4 (ROBO4 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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AP1muA single guide RNA

Plasmid

#230030

Purpose

guide RNA for AP1muA tagging at the C-terminus in pX459

Depositor

Francesca Bottanelli

Article

Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

Insert

AP1M1 guide (AP1M1 Human)

Use

CRISPR

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE

Plasmid

#89607

Purpose

Expression of LIN28A in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

LIN28A (LIN28A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

Plasmid

#66779

Purpose

The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

Depositor

Thomas Oertner

Article

Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

Inserts

hbeRhGC
red fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

human Synapsin

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a(cd226)

Plasmid

#177900

Purpose

Bacterial expression of his10-tagged human cd226 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

CD226 (aa 288-336 only) (CD226 Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-B-161;+80pCalb2

Plasmid

#66745

Purpose

luciferase reporter for Calb2 promoter (-161to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-RBPMS__NTRK3

Plasmid

#116836

Purpose

Lentiviral expression of RBPMS__NTRK3 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RBPMS__NTRK3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR[EF1α-copGFP]

Plasmid

#218327

Purpose

Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-SGK3__KRT9

Plasmid

#116837

Purpose

Lentiviral expression of SGK3__KRT9 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SGK3__KRT9

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

Plasmid

#175421

Purpose

Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pH4R-P2A-mCherry-N1

Plasmid

#84333

Purpose

Co-expresses (untagged) Histamine-4 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H4R-P2A-mCherry (HRH4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase gRNA resistant

Plasmid

#163411

Purpose

Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted. gRNA-t…

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2K930R-VSV

Plasmid

#139346

Purpose

Expression of kinase-dead TYK2-K930R protein

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-K930R

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-HTATSF1__BRS3

Plasmid

#116822

Purpose

Lentiviral expression of HTATSF1__BRS3 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

HTATSF1__BRS3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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