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Ypet-PBD

Plasmid

#105290

Purpose

To measure Rac or Cdc42 activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Pak1 (PAK1 Human)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 65-150

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Bmi1 shRNA1

Plasmid

#21576

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

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pIRES-Ap2a2-FKBP

Plasmid

#46944

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap2a2-FKBP

Tags

Mainly mouse Ap2a2 cDNA, with a fragment of brain…

Expression

Mammalian

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-VPS35-mScarlet

Plasmid

#215474

Purpose

Expression vector with VPS35 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

VPS35 (VPS35 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Actin Binding Domain Tensionless Control

Plasmid

#215371

Purpose

Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

Depositor

Brenton Hoffman

Article

Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

Actin Binding Domain Tensionless Control

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-UVRAG

Plasmid

#86738

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Tags

JA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDNR-SpCas9

Plasmid

#80425

Purpose

Used for in vitro transcription of SpCas9 mRNA.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Insert

SpCas9

Use

CRISPR

Tags

none

Mutation

codon optimized for human

Promoter

T7

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G3BP1-S149A

Plasmid

#135998

Purpose

S149A G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S149A

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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DYSF-Venus

Plasmid

#29768

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF-Venus (DYSF Human)

Tags

Venus

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Promoter

myo-3 and unc-119

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-Flag-RNF169

Plasmid

#74243

Purpose

Expresses RNF169 in mammalian cells

Depositor

Daniel Durocher

Article

Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

Insert

RNF169 (RNF169 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pMPMA3dPlac-null-GFPLVA

Plasmid

#23338

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
pBABE-hygro LAP2

Plasmid

#15740

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
CAG-Cremyc-2A-GFP

Plasmid

#116879

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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plexm-human LRRC33

Plasmid

#111600

Purpose

Express human LRRC33 in 393T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

LRRC33 (NRROS Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-RT007-GCN4scFV-VB

Plasmid

#163387

Purpose

2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

antiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)

Use

Lentiviral

Tags

BFP

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163073

Purpose

Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD63-(TEV)-tTA

Plasmid

#162597

Purpose

human CD63-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD63 (CD63 Human)

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-BFP

Plasmid

#153426

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

mTagBFP2

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBABE-puro LAP2

Plasmid

#15712

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only