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pILGFP92

Plasmid

#83549

Purpose

Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TDH3 promoter-yEGFP

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 I68A

Plasmid

#71864

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, Isoleucine 68 mutated to Alanine

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pFRT-TODestRFP_HuD

Plasmid

#65766

Purpose

expresses RFP-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Expression

Mammalian

Mutation

V23M mutation in RFP

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pRS416-PHO5-GFP-Osh1

Plasmid

#58835

Purpose

Expresses GFP-Tagged Osh1 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Osh1 (SWH1, OSH1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

PHO5

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pBSFI-p110γ

Plasmid

#34892

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110γ (PIK3CG Human)

Use

Adaptor plasmid

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
11.5kb-GFP (pBSK)

Plasmid

#29459

Depositor

Larry Rohrschneider

Article

Rohrschneider et al Dev Biol. 2005 Jul 15. 283(2):503-21.

Insert

SHIP-1 promoter (Inpp5d Mouse)

Tags

GFP

Expression

Bacterial

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
PKC epsilon dNPS

Plasmid

#21241

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC epsilon (Prkce Mouse)

Expression

Mammalian

Mutation

delta 1-163

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
PKC gamma dNPS

Plasmid

#21237

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Mutation

delta 1-28

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
rGSKmyc-R85-pXT7

Plasmid

#15493

Depositor

Sergei Sokol

Article

Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

Insert

GSK-3 beta R85 (Gsk3b Rat)

Use

Xenopus rna expression

Tags

myc

Mutation

R85: K85R, dominant negative

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pRK Smad3LC Flag

Plasmid

#14829

Depositor

Rik Derynck

Article

Zhang et al Curr Biol. 1997 Apr 1. 7(4):270-6.

Insert

Smad3 LC (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

amino acids 146-425

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
CIT96u

Plasmid

#128018

Purpose

K77A

Depositor

Uwe Sauer

Article

Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

Insert

CitA

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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B1-3

Plasmid

#111465

Purpose

translational release factor 3

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 3

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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B3-2

Plasmid

#111472

Purpose

translational elongation factor 4

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor 4

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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B5-5

Plasmid

#111484

Purpose

translational initiation factor 3

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 3

Tags

6xHis

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRL542

Plasmid

#70282

Purpose

ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_NT_AmpR

Plasmid

#119144

Purpose

Encodes non-targeting sgRNA

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

non-targeting sgRNA

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hY

Plasmid

#60995

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear citrine

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

citrine

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-pTH-iCre:EGFP-WPREpA

Plasmid

#213142

Purpose

Truncated rat TH promoter expressing iCre fused to EGFP

Depositor

Ulrik Gether

Article

Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

Insert

iCre

Use

AAV

Tags

EGFP

Expression

Mammalian

Promoter

Truncated TH promoter from rat (Rattus norvegicus…

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF3056

Plasmid

#144532

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

THRB (THRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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