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pCMV-myc3-HDM2

Plasmid

#20935

Depositor

Yue Xiong

Article

Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

Insert

Mdm2 p53 binding protein homolog (MDM2 Human)

Tags

myc3

Expression

Mammalian

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100849

Purpose

AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A2

Plasmid

#31100

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF4A (HNF4A Human)

Tags

Myc

Expression

Mammalian

Mutation

splice variant 2

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

Plasmid

#100846

Purpose

AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

Plasmid

#101178

Purpose

Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/M694A/Q695A/H698A

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

Plasmid

#218868

Purpose

Mammalian expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

CAG

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-pAceR-Kv2.1PR

Plasmid

#195534

Purpose

Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVTRE3_Clover

Plasmid

#135179

Purpose

AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Inserts

Clover
TRE3G

Use

AAV

Expression

Mammalian

Mutation

a variant of GFP

Promoter

TRE3G

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-G1-ATP8-Myc-FLAG (G418)

Plasmid

#86850

Purpose

mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP8 (ATP8 Human)

Tags

ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEf1a-superDn29-P2A-GFP

Plasmid

#247152

Purpose

Mammalian expression of a human codon optimized engineered superDn29 recombinase (highest efficiency variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1950

Plasmid

#205983

Purpose

Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Inserts

Ga0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array

Tags

14xHis and TwinStrep-tag

Expression

Bacterial

Promoter

T7 (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2-Flag

Plasmid

#180407

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-mTLR4ecd-VLR

Plasmid

#187877

Purpose

Baculovirus protein expression of mouse TLR4 (residues 26–544) fused with hagfish variable lymphocyte receptor (VLR) (residues 126–200)

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

TLR4ecd (Tlr4 Mouse)

Expression

Insect

Mutation

includes residues 26–544 of mouse TLR4

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP1-10

Plasmid

#65829

Purpose

For membrane contact

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Tags

spGFP1-10

Expression

Worm

Promoter

Prig-3

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK3

Plasmid

#122674

Purpose

Expresses mouse PCSK3 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEf1a-goldDn29-P2A-GFP

Plasmid

#247153

Purpose

Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100851

Purpose

AAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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lentiGuideFB-Puro-A

Plasmid

#192506

Purpose

For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2_C277S-Flag

Plasmid

#180412

Purpose

lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2_C277S (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C277S

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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