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Plasmid#147836PurposeMammalian Expression of HsNot1iso1-del1097-1110DepositorInsertHsNot1iso1-del1097-1110 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1-del1097-1110-shRNAres_W
Plasmid#147882PurposeMammalian Expression of HsNot1iso1-del1097-1110-shRNAresDepositorInsertHsNot1iso1-del1097-1110-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_162 SpCas9 KES(1107-1109)GG
Plasmid#179526PurposeFor bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 KES(1107-1109)GG
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationreplaced KES (residues 1107-1109) with GGPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
109_pAAV-ProC19-CatCh-GFP-WPRE
Plasmid#125954PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVTagsExpressionMutationPromoterProC19Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 N109-119 (#432)
Plasmid#99933Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationdelAA109-119insNAAIRS linkerPromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001146109)
Plasmid#80242Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-L108S/L109S/F112S
Plasmid#48730PurposeBacterial expression of Lacritin Protein with Mutation at proteins 108 and 109 Leucine to Serine and 112 from phenylalanine to serineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationLeucine 108 and 109 to Serine; phenylalanine 112 …PromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)
Plasmid#63139PurposeExpression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)DepositorUseTagsGSTExpressionBacterialMutationResidues 1-109 and Residues 1-159PromotertacAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]
Plasmid#149426PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q79N (Q109H) (TAC-->TAT)
Plasmid#105736PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q79N (Q109H) (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ79N/Q109HPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-Calbindin [L109/39-rat IgG2a] - Chimeric
Plasmid#227042PurposeMammalian expression plasmid of anti-Calbindin (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L109/39.DepositorInsertAnti-Calbindin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME TAEL2.0-5xC120-minpro (JDW 1109)
Plasmid#224507PurposeGateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)DepositorInsertTAEL2.0-5xC120
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Myc-BioID2-MCS1 (JDW 1095)
Plasmid#224510PurposeGateway compatible middle entry clone containing a Myc-tagged BIOID2 constructDepositorInsertMyc-BioID2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
anti-Calbindin scFv [L109/57] with sortase tag
Plasmid#204421PurposeMammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57.DepositorInsertRecombinant mouse scFv targeting Calbindin (Homo sapiens)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA_X
Plasmid#147999PurposeMammalian Expression of HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAADepositorInsertHsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_W
Plasmid#147881PurposeMammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAresDepositorInsertHsNot1iso1-del1097-1110-R1138A-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]
Plasmid#149427PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_X
Plasmid#148000PurposeMammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAADepositorInsertHsNot1iso1_840-1605-del1097-1110-RNFtoAAA (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q79N Q109N (TAC-->TAT)
Plasmid#105738PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ79N/Q109NPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-Laccase2 608-1097
Plasmid#69890PurposeExpresses the Drosophila laccase2 exon 2 circular RNADepositorInsertLaccase2 (stw Fly)
UseTagsExpressionInsectMutationL158I in laccase2PromoterMetallothionein Promoter (pMT)Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only