We narrowed to 5,868 results for: nls

1. CAGGS-SAFB-NLS-3XFLAG-V5

   Plasmid

   #196089

   Purpose

   Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR1a-jAspSnFR3-mRuby3-NLS

   Plasmid

   #203489

   Purpose

   Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3-mRuby3-NLS
   

   Use

   Gateway entry vector

   Tags

   Nuclear localization signal and mRuby3

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. iB22 PB-zim3-mycNLS-mApple

   Plasmid

   #204718

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MCS-T2a-GFPnls-ER-Cre-ER

   Plasmid

   #200104

   Purpose

   Vector For Twist

   Depositor

   Prayag Murawala

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Twist3cterm-GFPnls-T2A-ER-Cre-ER

   Plasmid

   #200105

   Depositor

   Prayag Murawala

   Insert

   Twist3:cTer-KI-eGFPnls-T2A-ER-Cre-ER
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. C-SWAT IAA17-6FLAG (no NLS)

   Plasmid

   #193322

   Purpose

   C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   IAA17
   

   Use

   Tags

   6FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-NLS-MaSat ZF-18-mVenusN

   Plasmid

   #191392

   Purpose

   Zinc finger fused to N-terminal part of split mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   Flag-NLS-MaSat ZF-18-mVenusN
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV2-UbC-3xNLS-mScarlet-I

   Plasmid

   #191099

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Pantelis Tsoulfas, Murray Blackmore

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet-I
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   optimized to human codon usage

   Promoter

   UbC

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-3x-NLS-mScarlet-AMPKα2

   Plasmid

   #192452

   Purpose

   mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
   

   Use

   Tags

   3x Nuclear localization signal (NLS) and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-EF1a-ArgSen (-)NLS

    Plasmid

    #184689

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-EF1a-ArgSen (-)NLS

    Plasmid

    #184698

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. nPPARg429-gs10-NLS-VPR

    Plasmid

    #188266

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nPPARg429 (PPARG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENT-L1-Kozak-NLSlacZ-stop-L2

    Plasmid

    #186358

    Purpose

    Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ Synthetic, E. coli)
    

    Use

    Expression of nuclear lacz reporter

    Tags

    Nuclear localization signal

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 2xNLS-NLP-cMyc AspCas12a

    Plasmid

    #182120

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH9-Tier1-PhCMV-3xNLS

    Plasmid

    #169478

    Purpose

    Tier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven nuclear localization signal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH8-Tier1-PhCMV-NLS

    Plasmid

    #169477

    Purpose

    Tier-1 vector encoding PhCMV-driven nuclear localization signal expression (PhCMV-NLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven nuclear localization signal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. NLS-tdPCP-GFP-VP16

    Plasmid

    #183935

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP-VP16 (UL48 Synthetic)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-NLS-YFP-CCTc

    Plasmid

    #184327

    Purpose

    Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)

    Depositor

    Xiaodong Liu

    Article

    Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

    Insert

    CCTc (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-PAG-E2-Crimson-3XNLS-pA

    Plasmid

    #183801

    Purpose

    AAV vector to express nuclear localized E2-Crimson from PAG promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    E2-Crimson-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV_Hyg_FLAG-BirA-eGFP-NLS2

    Plasmid

    #170917

    Purpose

    Expresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull down

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    FLAG-BirA-eGFP-NLS2
    

    Use

    Tags

    BirA-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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