We narrowed to 9,182 results for: Pol;

1. pFRT-TO-RPB1-His K710,719R CRres si2,4R

   Plasmid

   #139407

   Purpose

   Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K710,719R

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

   Plasmid

   #176236

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176237

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176238

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. wtVP1 + VP2C-GFPdeg

   Plasmid

   #166678

   Purpose

   Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFPdeg
   Wild-type Murine polyomavirus VP1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. deltaVP1 + VP2C-GFPdeg

   Plasmid

   #166677

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFPdeg
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-Neo-NCOA7g3 (BB36)

   Plasmid

   #139454

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide-Neo-NCOA7g2 (BB35)

   Plasmid

   #139453

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUDP046

   Plasmid

   #107062

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting KU80 from Ogataea parapolymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting OpKU80 inO. parapolymorpha
   

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5b HA Irx5 deltaIrot

    Plasmid

    #24995

    Depositor

    Benoit Bruneau

    Article

    Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

    Insert

    Irx5 (Irx5 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of Iro box (aa 314-327), 19 aa upstream …

    Available Since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pCMV5b HA Irx5 deltaC

    Plasmid

    #24996

    Depositor

    Benoit Bruneau

    Article

    Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

    Insert

    Irx5 (Irx5 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletes everything after Hox Domain (aa 112-177).…

    Available Since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-21b(+)-Is-PETase-W185A

    Plasmid

    #112204

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W185A

    Promoter

    N/A

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUltra-Hot-PC

    Plasmid

    #184466

    Purpose

    Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS2181

    Plasmid

    #158421

    Purpose

    AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple32 (CLDN5 Human)
    

    Use

    AAV

    Promoter

    Ple32

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

    Plasmid

    #139444

    Purpose

    Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    sequence codon-optimized

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. BCL2alpha-MJK2243

    Plasmid

    #227690

    Purpose

    Expresses human BCL2alpha

    Depositor

    Curt Civin

    Article

    Goodis et al Eur J Med Chem. 2024 Dec 22;285:117190.

    Insert

    human BCL-2alpha (BCL2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

    Plasmid

    #156431

    Purpose

    Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    RAD21, Halotag (Rad21 Mouse)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUDP013

    Plasmid

    #103873

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C

    Plasmid

    #114613

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, A60C; 23 polyQ repeat

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTWIN1-His6-Ssp-Htt2-90-43Q-P90C

    Plasmid

    #114626

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 43 polyQ repeat

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTWIN1-His6-Ssp-Htt2-90-15Q-P90C

    Plasmid

    #114611

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 23 polyQ repeat

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q

    Plasmid

    #114617

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 23 polyQ repeat

    Available Since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTWIN1-His6-Ssp-Httex1-43Q-A2C

    Plasmid

    #114622

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 43 polyQ repeat

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTWIN1-His6-Ssp-Httex1-43Q-A2C-A60C

    Plasmid

    #114623

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, A60C; 43 polyQ repeat

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q

    Plasmid

    #114627

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 49 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 49 polyQ repeat

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTWIN1-His6-Ssp-Htt2-90-49Q-P90C

    Plasmid

    #114631

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 49 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 49 polyQ repeat

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSN-A112C-1A32

    Plasmid

    #191241

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSN-A112C-1A31

    Plasmid

    #191242

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSN-A112C-CP8B1

    Plasmid

    #191240

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge