We narrowed to 2,304 results for: tub

1. pCAG-DIO-basic

   Plasmid

   #196882

   Purpose

   Used for preparation of double-floxed inverse ORF vectors

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Type

   Empty backbone

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBactin-mOrange2-CI

   Plasmid

   #196852

   Purpose

   Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DsRed -7e

   Plasmid

   #191765

   Purpose

   Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Use

   Tags

   N terminal fusion of negatively charged polypetod…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7gRNA-smyhc1_977

   Plasmid

   #140867

   Purpose

   sgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   zebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Synthetic, Zebrafish)
   

   Use

   In vitro transcription of sgrnas

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT7gRNA-ttn.1_C3

   Plasmid

   #140866

   Purpose

   sgRNA synthesis vector for ttn.1_C3 (zebrafish titin .1).

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   zebrafish ttn.1_C3 sgRNA for in vitro transcription (ttn.1 Synthetic, Zebrafish)
   

   Use

   In vitro transcription of sgrnas

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMtb MCM

   Plasmid

   #138296

   Purpose

   Expression of Mtb MCM small and large subunit

   Depositor

   Ruma Banerjee

   Article

   Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

   Inserts

   mutA
   mutB
   

   Use

   Tags

   His tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   inserted TEV cleavage site between His-tag and m…

   Promoter

   T7

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p3E-Cltb

   Plasmid

   #109586

   Purpose

   Multisite gateway vector for 3' tagging with Clathrin light chain b

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Clathrin light chain b (cltb Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3E-jph1a

   Plasmid

   #109551

   Purpose

   Multisite gateway vector for 3' tagging with junctophilin 1a

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   junctophilin 1a (jph1a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Please see Depositor Comments

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3E-ARF1

   Plasmid

   #109589

   Purpose

   Multisite gateway vector for 3' tagging with ARF1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   ARF1 (arf1 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3E-ARF6

    Plasmid

    #109591

    Purpose

    Multisite gateway vector for 3' tagging with ARF6

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF6 (arf6a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pME-Cav3

    Plasmid

    #109570

    Purpose

    Multisite gateway vector for 5' tagging with Caveaolin3

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    caveolin3 (cav3 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p3E-Lact-C2

    Plasmid

    #109553

    Purpose

    Multisite gateway vector for 3' tagging with the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8).
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. actc1b-mKate2-Dnm2a

    Plasmid

    #109519

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to Dynamin2a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Dnm2a (dnm2a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. actc1b-mKate2-EHD1b-dEH

    Plasmid

    #109515

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to EHD1b-delta-EH

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    EHD1b (ehd1b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    dEH

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

    Plasmid

    #124066

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-R232Q-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    R232Q

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-hygro-eGFP-STOP-HEC1

    Plasmid

    #114026

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-STOP-HEC1 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Start codon of Hec1 is mutated to stop codon

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac-LacI-LAP-HEC1

    Plasmid

    #114033

    Purpose

    Production of baculovirus with LacI-LAP-HEC1

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    LacI-LAP-HEC1 (NDC80 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSG5 N-HA SADBs shRNA resistant

    Plasmid

    #67157

    Purpose

    N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    Brsk1 (Brsk1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…

    Promoter

    SV40 promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pVRa40_3198

    Plasmid

    #49701

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF40_3198
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVRa14_3200

    Plasmid

    #49666

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF14_3200
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFM038

    Plasmid

    #40141

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    R183-5A + 7SA; base pairs 6-523 of par-2 were re…

    Promoter

    pie-1

    Available since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pFM041

    Plasmid

    #40144

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (S241A) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    S241A; base pairs 6-523 of par-2 were recoded to…

    Promoter

    pie-1

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pFM039

    Plasmid

    #40142

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    R183-5A + S241A; base pairs 6-523 of par-2 were …

    Promoter

    pie-1

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pFM036

    Plasmid

    #40139

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (K162A) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    K162A; base pairs 6-523 of par-2 were recoded to…

    Promoter

    pie-1

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pFM052

    Plasmid

    #40146

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (C56S) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    C56S; base pairs 6-523 of par-2 were recoded to …

    Promoter

    pie-1

    Available since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pFM037

    Plasmid

    #40140

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (R163A) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    R163A; base pairs 6-523 of par-2 were recoded to…

    Promoter

    pie-1

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pBactin-mNeonGreen-CI

    Plasmid

    #196850

    Purpose

    Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCherry

    Plasmid

    #176016

    Purpose

    Mammalian expression of mCherry. Parton lab clone DFQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP

    Plasmid

    #176015

    Purpose

    Mammalian expression of EGFP. Parton lab clone DFS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-Kif2A

    Plasmid

    #52401

    Purpose

    Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

    Depositor

    Gohta Goshima, Ryota Uehara

    Article

    Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

    Insert

    Kif2A (KIF2A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge