We narrowed to 12,075 results for: HAL;

1. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

   Plasmid

   #86843

   Purpose

   HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

   Depositor

   Thomas Cech

   Article

   Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

   Insert

   FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
   

   Tags

   FLAG-HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous TERT promoter

   Available Since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

   Plasmid

   #164614

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.

   Depositor

   Jeffrey Chao

   Article

   Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

   Insert

   stdMCP-stdHalo-RH1
   

   Use

   Lentiviral

   Tags

   HA tag and NLS

   Expression

   Bacterial and Mammalian

   Promoter

   UbiC

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-GRAB-HaloDA1.0-IRES-EGFP-CAAX

   Plasmid

   #234410

   Purpose

   Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

   Insert

   GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDisplay-GRAB-HaloDAmut-IRES-EGFP-CAAX

   Plasmid

   #234411

   Purpose

   Expresses the chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

   Insert

   GPCR activation based chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-SKII-URA-GAL1-HALO-3xHA

   Plasmid

   #188937

   Purpose

   Integration cassette to create N-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   URA-GAL1-HALO-3xHA
   

   Expression

   Yeast

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. R713-M85-303: CMV51p> Halotag-Hs.KRAS4a

   Plasmid

   #159680

   Purpose

   Mammalian protein expression of KRAS4a-Halotag fusion

   Depositor

   Dominic Esposito

   Article

   Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

   Insert

   Halotag-Hs.KRAS4a (KRAS Human)
   

   Tags

   Halotag

   Expression

   Mammalian

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

   Plasmid

   #202571

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFSW GAP43-jRGECO1a P2A Syp-Halo

   Plasmid

   #185565

   Purpose

   Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

   Insert

   GAP43-jRGECO1a P2A Syp-Halo
   

   Use

   Lentiviral

   Tags

   HaloTag

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)

   Plasmid

   #208395

   Purpose

   To generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   Human MRE11 (MRE11 Human)
   

   Use

   Luciferase and Retroviral

   Expression

   Mammalian

   Mutation

   DB1delta/DB2delta

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

    Plasmid

    #197066

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    CRISPR

    Tags

    FKBP and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

    Plasmid

    #134611

    Purpose

    Mammalian expression of mouse KIF5A 378-1027

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5A (Kif5a Mouse)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

    Plasmid

    #134613

    Purpose

    Mammalian expression of rat KIF5C 378-955

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5C (Kif5c Rat)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Halo-Tag-128-425-Akap9

    Plasmid

    #196873

    Purpose

    Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Halo-Tag-Akap9 (128-425) (Akap9 Rat)
    

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139485

    Purpose

    PCR template for Halo-Tag gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    HaloTag-loxP-P2A-dTKneo-pA-loxP
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

    Plasmid

    #198599

    Purpose

    Green fluorescent Ca2+ ion indicator for bacterial expression.

    Depositor

    Robert Campbell

    Article

    Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

    Insert

    HaloGFP-143-144
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

    Plasmid

    #134612

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 375 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid

    #215001

    Purpose

    Shuttling vector for a mitophagy reporter

    Depositor

    Wade Harper

    Insert

    pSu9 (Presequence of Fo-ATPase subunit 9)
    

    Use

    Gateway

    Tags

    HaloTag(7)-mGFP

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-BLAST Halo-flag-CAD

    Plasmid

    #188117

    Purpose

    Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag and Halo tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

    Plasmid

    #202568

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

    Plasmid

    #175421

    Purpose

    Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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