We narrowed to 9,675 results for: SUB;

1. pECE-M2-PPP1R12C wt

   Plasmid

   #31659

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   Protein Phosphatase 1 Regulatory Subunit 12C (PPP1R12C Human)
   

   Tags

   M2

   Expression

   Mammalian

   Mutation

   codon-optimized

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

   Plasmid

   #226350

   Purpose

   Expression of proteasome substrate with tail for molecular ruler

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBiex PKC - Peptide 8 GFP RFP

   Plasmid

   #83564

   Purpose

   Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PKC alpha (PRKCA Human)
   

   Tags

   10 nm ER/K Helix, FLAG purification tag, TEV Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pECE HA AMPKa1 AS

   Plasmid

   #69831

   Purpose

   Expresses Expresses AMPK alpha 1 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   SV40 early promoter

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMito-LSSmKate2-N1

   Plasmid

   #31879

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   human cytochrome C oxidase subunit VIII (COX8A Human)
   

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

   Available Since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. FastLightR-bRaf-mVenus

   Plasmid

   #162155

   Purpose

   Expresses FastLightR-bRaf-mVenus in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   FastLightR-bRaf-mVenus
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Valine 600 is substituted with Glutamic acid

   Promoter

   CMV

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFudioFRTPSD-95TealW

   Plasmid

   #134299

   Purpose

   Expresses PSD95 fused to Teal fluorescent protein upon coexpression with the recombinase flippase (Flp)

   Depositor

   Elly Nedivi

   Article

   Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

   Insert

   PSD-95-Teal (Dlg4 Rat, Mouse)
   

   Use

   Lentiviral; Flp/frt

   Tags

   Teal

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMX-SHP1C-ires-hCD8

   Plasmid

   #183720

   Purpose

   Expresses the myc-tagged catalytic domains of wild-type SHP-1.

   Depositor

   Takeshi Tsubata

   Article

   Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

   Insert

   SHP-1
   

   Use

   Retroviral

   Mutation

   catalitic domain alone

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LightR-bRaf-mVenus

   Plasmid

   #162154

   Purpose

   Expresses LightR-bRaf-mVenus in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   LightR-bRaf-mVenus
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Valine 600 is substituted with Glutamic acid

   Promoter

   CMV

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBiex PKC - Peptide 8 mCit mCer

    Plasmid

    #83563

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PRKCA (PRKCA Human)
    

    Tags

    10 nm ER/K Helix, FLAG purification tag, TEV Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

    Plasmid

    #113952

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, I132S and S212N su…

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-HA-Flag-natT1R2 FS10

    Plasmid

    #113956

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, S212N substitution…

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCHECK-2-TGFBR2-m3_3'UTR

    Plasmid

    #31883

    Depositor

    Robert Blelloch

    Article

    Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

    Insert

    TGFBR2 mutant 3'UTR (TGFBR2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Third miR-302b, 372 7-mer seed match mutated from…

    Promoter

    SV40

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCEP4-HA-Flag-natT1R2 a22-end_S212N

    Plasmid

    #113951

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, S212N substitution

    Promoter

    CMV

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CFP-Rpp29-C1

    Plasmid

    #134546

    Purpose

    Expresses CFP-Rpp29 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Rpp29 (POP4 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBX_RPL29_smGFP_V5

    Plasmid

    #236091

    Purpose

    Constitutively encodes RPL29-smGFP-V5 (sfGFP-based spaghetti monster fluorescent protein embedding V5 epitope tags), for targeting RPL29 in the 60S subunit.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    RPL29-smGFP-V5 (RPL29 Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHPHS926

    Plasmid

    #228237

    Purpose

    Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing pad

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

    Inserts

    mCherry
    Beta globin
    

    Tags

    3xFLAG, HA, and PuroR

    Expression

    Mammalian

    Mutation

    PTC39

    Promoter

    TRE3GV and none

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

    Plasmid

    #226349

    Purpose

    Expression of Titin V15P substrate with GS tail

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Expression

    Bacterial

    Mutation

    V15P

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10

    Plasmid

    #226351

    Purpose

    Expression of Titin (V15P) substrate for 26S proteasome

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Expression

    Bacterial

    Mutation

    V15P

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5

    Plasmid

    #226352

    Purpose

    Expression of Titin (V15P) substrate for 26S proteasome

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Expression

    Bacterial

    Mutation

    V15P

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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