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  2. NESRA-DEVD-BNLS

    Plasmid
    #50849
    Purpose
    Expresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Use
    Tags
    NES sequence LQKKLEELELDE placed after ddGFP A an…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    50849-plasmid-map-sequence-id-76294

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  10. RANLS-IETD-BNES

    Plasmid
    #60972
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Use
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA-LmoCas6-p300

    Plasmid
    #126486
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Insert
    LmoCas6-p300
    Use
    CRISPR
    Tags
    Flag and Flag, NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126486-plasmid-map-sequence-id-246633

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  14. pcDNA-CasE-p300-Flag

    Plasmid
    #106275
    Purpose
    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Insert
    EcoCasE-p300
    Use
    CRISPR
    Tags
    E tag, NLS and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    106275-plasmid-map-sequence-id-247907

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  18. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

    Plasmid
    #222288
    Purpose
    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.
    Depositor
    Anne Straube
    Article
    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-
    Insert
    KIF1C (KIF1C Human)
    Use
    Tags
    6xHis, GFP, and GST
    Expression
    Bacterial
    Mutation
    stalk region only amino acids 642 to 922
    Promoter
    T7
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222288-plasmid-map-sequence-id-475373

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Showing: 1061 - 1080 of 21255 results