We narrowed to 21,676 results for: Tes

1. pTwist-WIV1-CoV Δ18

   Plasmid

   #164439

   Purpose

   Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   WIV1-CoV Spike truncated to remove 18 amino acids
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-NES-NIR-GECO1

   Plasmid

   #113683

   Purpose

   Express NIR-GECO1 preferentially in neurons

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NES-NIR-GECO1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. NESRA-DEVD-BNLS

   Plasmid

   #50849

   Purpose

   Expresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A and ddRFP B
   

   Use

   Tags

   NES sequence LQKKLEELELDE placed after ddGFP A an…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. single polypeptide FPX biosensor for caspase-8

   Plasmid

   #60884

   Purpose

   FPX biosensor for caspase-8

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-8
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pBAD-K-GECO1

   Plasmid

   #105864

   Purpose

   Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

   Depositor

   Robert Campbell

   Article

   Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

   Insert

   K-GECO1
   

   Use

   Tags

   6-His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTwist-WIV1-CoV

   Plasmid

   #164438

   Purpose

   Encodes WIV1-CoV Spike Protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   WIV1-CoV Spike
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINK-OTUD4 (OTU, aa 22-196)

   Plasmid

   #61413

   Purpose

   Expresses human OTUD4 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-21 and aa 197-1114.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. single polypeptide FPX biosensor for both caspase-3 and -8

   Plasmid

   #60886

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-3 and 8
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

   Plasmid

   #32008

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   Lipin1 (Lpin1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

   Promoter

   CMV, SP6

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pRK5-HA-Ubiquitin-K27R-K29R

    Plasmid

    #121156

    Purpose

    Mammalian expression of HA tagged ubiquitin K27R and K29R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K27 and K29 mutated to Rs

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. RANLS-IETD-BNES

    Plasmid

    #60972

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pNL3.1-Erg

    Plasmid

    #182706

    Purpose

    Express Erg enhancer in mammalian cells

    Depositor

    Robert Rowe

    Article

    Wang et al Cell Rep. 2022 Apr 5;39(1):110587. doi:

    Insert

    Enhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-LmoCas6-p300

    Plasmid

    #126486

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6-p300
    

    Use

    CRISPR

    Tags

    Flag and Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag‐mTim3 (del.Cyto)

    Plasmid

    #49209

    Purpose

    expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (del.Cyto) (Havcr2 Mouse)
    

    Use

    Tags

    CD8 signal sequence and FLAG

    Expression

    Mammalian

    Mutation

    cytosolic domain deleted, no Tim3 signal sequence…

    Promoter

    Ef1a

    Available since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-CasE-p300-Flag

    Plasmid

    #106275

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE-p300
    

    Use

    CRISPR

    Tags

    E tag, NLS and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag‐mTim3 (T2 truncation)

    Plasmid

    #49211

    Purpose

    expresses Flag tagged mouse Tim3 (T2 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T2 truncation (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T2 truncation ( lacks all but one tyrosine, which…

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. KRAS-V14I

    Plasmid

    #154427

    Purpose

    V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

    Depositor

    Kenneth Westover

    Article

    Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag‐mTim3 (T1 truncation)

    Plasmid

    #49210

    Purpose

    expresses Flag tagged mouse Tim3 (T1 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T1 truncation (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T1 truncation ( contains all but the three most C…

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn1-DIO-ExRai-AKAR2-T/A

    Plasmid

    #171847

    Purpose

    Cre-dependent, synapsin promoter-driven expression of the negative control phosphomutant ExRai-AKAR2 T/A PKA biosensor in neurons.

    Depositor

    Richard Huganir

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2 T/A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    T6A phospho-deficient mutation

    Promoter

    hSyn1

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn1-DIO-ExRai-AKAR2

    Plasmid

    #171846

    Purpose

    Cre-dependent, synapsin promoter-driven expression of the green fluorescent ExRai-AKAR2 PKA biosensor in neurons.

    Depositor

    Richard Huganir

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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