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pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128344

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb3 (Chrnb3 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase-P2A-H2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

Plasmid

#229840

Purpose

A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128341

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna6 (Chrna6 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

Plasmid

#159630

Purpose

AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

Depositor

Ross Bathgate, Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

AAV

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Mutation

Promoter

DCA (same as CAG promoter)

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4

Plasmid

#193295

Purpose

episomal vector expressing mCherry + human SOX2A61V + KLF4

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry-t2a-SOX2AV-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)

Use

Tags

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

Plasmid

#171379

Purpose

Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized Cas13X.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

Plasmid

#193294

Purpose

episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)

Use

Tags

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFB-U6-beta2 RD-1-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128343

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb2 (Chrnb2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

Plasmid

#200634

Purpose

Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

PTEN (PTEN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

Plasmid

#214471

Purpose

Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Inserts

ZFYVE27 (ZFYVE27 Human)
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S14D S25D S32D T261D

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-VGAT sesRNA-2a-msFlag-2a-tTA2-WPRE

Plasmid

#239030

Purpose

Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse VGAT (Slc32a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-Satb2 sesRNA-2a-msFlag-2a-tTA-WPRE

Plasmid

#239028

Purpose

Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse Satb2 (Satb2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171595

Purpose

Weak human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynI-mCherry-F2A-TVA-T2A-RabG-WPRE-hGH poly (A) (Alex_03)

Plasmid

#71654

Purpose

AAV expression of mCherry, TVA and RabG in human and mouse neurons for rabies mono-synaptic transfer experiments

Depositor

Mirjana Maletić-Savatić

Insert

F2A-TVA-T2A-RabG

Use

AAV; Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

hSynI

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-MUT-MRE

Plasmid

#235148

Purpose

Expresses the mCherry-based mutated (control) sensor for miR-124. TagBFP2 is expressed as a transduction reporter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mCherry with a mutated miR-124 MRE

Use

AAV

Tags

Expression

Mutation

7 nucleotides of the miR-124 WT MRE mutated

Promoter

EFS

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-Rorb sesRNA-2a-msFlag-2a-tTA2-WPRE

Plasmid

#239029

Purpose

Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse Rorb (Rorb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

Plasmid

#108362

Purpose

Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

Depositor

Luisa Cochella

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Inserts

Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
mCherry
his-15 (his-15 Nematode)
unc-54 3' UTR

Use

Tags

2xFlag, MYC, T2A peptide, and TY1 linker

Expression

Worm

Mutation

Promoter

A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct

Plasmid

#175552

Purpose

Targeting vector to introduce an FKBPV-HA2-P2A-mCherry cassette at the mouse SOX2 locus. Degradation Tag (dTAG) system.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

SOX2 (Sox2 Mouse)

Use

Mouse Targeting

Tags

FKBPV-HA2-P2A-mCherry

Expression

Mammalian

Mutation

none

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

Plasmid

#184381

Purpose

This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Cas9, two gRNAs targeting dystrophin

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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