We narrowed to 5,516 results for: pcas
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Plasmid#194204PurposeCre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.DepositorInsertRas Homolog Enriched Protein in Brain (S16H) (RHEB Human)
UseCre/LoxTagsExpressionMammalianMutationS16HPromoterAvailable sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MP-84-spCas9-U6-gRNA
Plasmid#206935PurposeExpression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.DepositorInsertspCas9
UseAAVTagsSV40 NLSExpressionMammalianMutationPromoterMP-84Available sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_NPM1_G6
Plasmid#178091PurposeExpresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.DepositorInsertNPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-5HA-mISG15
Plasmid#12444DepositorInsertISG15
UseTags5xHAExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCA24N_6xHis-TEV-rpsA
Plasmid#83585PurposeExpress E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tagDepositorInsertrpsA (rpsA Escherichia coli)
UseTagsHis tagExpressionBacterialMutationPromoterp-T5 LacAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a-PcAad1
Plasmid#163039Purposeexpression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporiumDepositorInsertaryl-alcohol dehydrogenase
UseTagsGST and His6ExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6a-V106W
Plasmid#215824PurposeExpress CBE6a-V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6a V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 AMSH 1-178
Plasmid#160050PurposeExpresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.DepositorInsertAMSH residues 1-178 (STAMBP Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-178PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-HygR
Plasmid#118153PurposeSpCas9 expression vectorDepositorInserthSpCas9-T2A-HygR
UseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCAG and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6a-IVT
Plasmid#215833PurposeTemplate for in vitro transcription of CBE6a (with SpCas9)DepositorInsertCBE6a-SpCas9-2xUGI
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pCadBA-mCherry
Plasmid#192865PurposepMUT2 based vector for sensing pH in E. coli Nissle 1917DepositorInsertpH sensitive promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 pHfluorin pCAGGS
Plasmid#206073Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial pHfluorin tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PPP1CB-miRFP703
Plasmid#178524PurposeExpresses PP1c-miRFP703 in mammalian cellsDepositorInsertPP1c-miRFP703 (PPP1CB Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3CC-FLAG
Plasmid#179162PurposeExpression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_LMNA_G2
Plasmid#178090PurposeExpresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.DepositorInsertLMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 blast
Plasmid#104997PurposeThis lentiviral construct delivers hSpCas9 and blasticidin S resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA
Plasmid#86613PurposeVector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmidDepositorInsertATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRha-ABE7.10-SpCas9-NG
Plasmid#170663PurposeExpresses the base editor ABE7.10-SpCas9-NG in bacterial cellsDepositorInsertABE7.10-SpCas9-NG
UseTags8xHIS and BP-NLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HiBiT-CD63
Plasmid#162591PurposeN-terminally HiBiT-tagged human CD63 under CAG promoterDepositorInserthuman CD63 (CD63 Synthetic, Human, human)
UseTagsHiBiTExpressionMammalianMutationPromoterCAG promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-EGFP-SOS1
Plasmid#178860PurposeMammalian expression of SOS1 fused to EGFPDepositorInsertSOS1 (SOS1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only