We narrowed to 5,093 results for: puc

1. pGL0_152 [pBP1 ORI]

   Plasmid

   #210674

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Insert

   pBP1 ORI
   

   Use

   Synthetic Biology

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AgU6.695&557_3xP3-DsRed1-SV40 (Tandem 1_2 gRNAs)

   Plasmid

   #243012

   Purpose

   For cloning two gRNAs in tandem after BspQI digestion under An. gambiae U6.695 and U6.557 promoters.

   Depositor

   George Christophides

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Insect

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRISPR plasmid

   Plasmid

   #236553

   Purpose

   Encodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. StarShip01

   Plasmid

   #164505

   Purpose

   To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.

   Depositor

   Bo Huang

   Type

   Empty backbone

   Use

   Bxbi site specific integration

   Availability

   Academic Institutions and Nonprofits only

   

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5. StarShip02

   Plasmid

   #164506

   Purpose

   To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.

   Depositor

   Bo Huang

   Type

   Empty backbone

   Use

   Bxbi site specific integration

   Availability

   Academic Institutions and Nonprofits only

   

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6. StarShip03

   Plasmid

   #164507

   Purpose

   To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.

   Depositor

   Bo Huang

   Type

   Empty backbone

   Use

   Bxbi site specific integration

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExp-RBD-CHis

   Plasmid

   #195002

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Article

   Fischer et al bioRxiv 2023.01.10.523494

   Insert

   RBD (S SARS-CoV-2)
   

   Tags

   His8

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-dSa-VPR

   Plasmid

   #99651

   Purpose

   Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9

   Depositor

   George Church

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VP64-p65-RTA activator

   Mutation

   dead Cas9

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sperm whale Mb 64Y68F in pVP80K

   Plasmid

   #86956

   Purpose

   Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

   Depositor

   John Olson

   Insert

   Mb 64Y68F
   

   Expression

   Bacterial

   Mutation

   64Y68F

   Promoter

   T5

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AgU6.695&MosU6.syn_3xP3-DsRed1-SV40 (Tandem 2_2 gRNAs)

    Plasmid

    #243013

    Purpose

    For cloning two gRNAs in tandem after BspQI digestion under An. gambiae U6.695 and U6.MosU6syn (synthetic mosquito U6) promoters.

    Depositor

    George Christophides

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Insect

    Available Since

    Sept. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExp-His-zBasic-RBD-Avi

    Plasmid

    #195000

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Article

    Fischer et al bioRxiv 2023.01.10.523494

    Insert

    RBD (S SARS-CoV-2)
    

    Tags

    Avi-tag and His8-Zbasic

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINT3

    Plasmid

    #24778

    Depositor

    Tanya Parish

    Article

    Parish et al Tuberculosis (Edinb). 2005 May . 85(3):1

    Type

    Empty backbone

    Use

    Integrating vector

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pULZ

    Plasmid

    #169813

    Purpose

    Production of circular recombinant RNA in Escherichia coli using viroid scaffolds, RNA precursor expression.

    Depositor

    Jose-Antonio Daros

    Article

    Daros et al Methods Mol Biol. 2021;2323:99-107. doi:

    Type

    Empty backbone

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. T7-3e5

    Plasmid

    #106990

    Purpose

    T7 transcription template for 3e5

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    3e5
    

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. T7-5i3e5

    Plasmid

    #106995

    Purpose

    T7 transcription template for 5i3e5

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    5i3e5
    

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas9-T2A-H2B mCitirine

    Plasmid

    #210471

    Purpose

    Coexpression of Cas9 and H2B mCitrine

    Depositor

    Amro Hamdoun

    Insert

    Cas9-T2A-H2B mCitirine
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mCitrine on C terminal of H2B

    Expression

    Mammalian

    Mutation

    Cas9 contains 3xFLAG tag and nuclear localization…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRHA40

    Plasmid

    #138941

    Purpose

    XOR gate with IPTG and aTc as inputs and mRFP1 as output

    Depositor

    Guillermo Rodrigo

    Article

    Rosado et al PLoS Genet. 2018 Jul 19;14(7):e1007548.

    Insert

    riboregulated mRFP1
    

    Expression

    Bacterial

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7-5iX

    Plasmid

    #106991

    Purpose

    T7 transcription template for 5iX

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    5iX
    

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. T7-Xi3

    Plasmid

    #106992

    Purpose

    T7 transcription template for Xi3

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    Xi3
    

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7-3eX

    Plasmid

    #106993

    Purpose

    T7 transcription template for 3eX

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    3eX
    

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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