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pCDH_Str-KDEL_ManII-SBP-mCherry

Plasmid

#65259

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FLAG-HA-mRFP-pcDNA3.1-

Plasmid

#52510

Purpose

expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

mRFP

Tags

FLAG-HA

Expression

Mammalian

Mutation

engineered variant of tetrameric red fluorescent …

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mIFP-Mito-7

Plasmid

#56231

Purpose

Localization: Mitochondria, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Publication

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mito (COX8A Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pJZC638

Plasmid

#62283

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Nuclease activity has been deactivated by mutatio…

Promoter

pAdh and pGal10

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMU-TGN41r

Plasmid

#61176

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pTEF1p-CYC1t

Plasmid

#62190

Purpose

Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Publication

Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

Insert

3xFLAG-dCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Yeast

Mutation

human codon-optimized, D10A, H840A

Promoter

TEF1 promoter

Available since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArA WT

Plasmid

#59804

Purpose

Allows the integration of Myc ArA in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Publication

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora A (AURKA Human)

Tags

Myc

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMU-PLAr

Plasmid

#61200

Purpose

Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRubisco1sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Publication

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RAB14 WT

Plasmid

#61493

Purpose

to overexpress wild type RAB14. Vector includes GFP driven by hUbC promoter

Depositor

Curt Civin

Publication

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLX304-YAP1 _SH3bm

Plasmid

#59141

Purpose

YAP1 mutants that harbored a deletion of SH3 binding motif, which disrupts interaction of YES1 with YAP1

Depositor

William Hahn

Publication

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

SH3 domain deleted (aa278-290)

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pVITRO-HPV53 L1L2

Plasmid

#52599

Purpose

Expresses HPV53 L1 and L2 in mammalian cells

Depositor

Richard Roden

Publication

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV53 L1
HPV53 L2

Expression

Mammalian

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV52 L1L2

Plasmid

#52584

Purpose

Expresses HPV52 L1 and L2 in mammalian cells

Depositor

Richard Roden

Publication

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV52 L1
HPV52 L2

Expression

Mammalian

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-CTR1

Plasmid

#53157

Purpose

Expresses human CTR1 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Publication

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

Retroviral

Expression

Mammalian

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX4T3-HA-ERK2 K54R

Plasmid

#53170

Purpose

Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector

Depositor

Christopher Counter

Publication

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Tags

GST and HA

Expression

Bacterial

Mutation

K54R

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only
pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV6 L1L2

Plasmid

#52589

Purpose

Expresses HPV6 L1 and L2 in mammalian cells

Depositor

Richard Roden

Publication

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV6 L1
HPV6 L2

Expression

Mammalian

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV8 L1L2

Plasmid

#52598

Purpose

Expresses HPV8 L1 and L2 in mammalian cells

Depositor

Richard Roden

Publication

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV8 L1
HPV8 L2

Expression

Mammalian

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2

Plasmid

#51250

Purpose

Encodes N-3xHA-TEV-mouseGli2-C

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRSM3

Plasmid

#49799

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Publication

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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