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EcAvs2

Plasmid

#188789

Purpose

Avs2 from Escherichia coli NCTC9087

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL11197

Plasmid

#191784

Purpose

SpCas9 expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas9 +13 introns-T-E9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pERB109

Plasmid

#58280

Purpose

constitutive miRFKBP5_Mis12-GFP-FKBP3; inducible mCh-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

FKBP-targeted miRNA
Mis12-GFP-FKBPx3
inducible mCh-FRB

Tags

GFP and mCherry

Expression

Mammalian

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-hphMX6

Plasmid

#105161

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-hphMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mOrange2

Plasmid

#96938

Purpose

Retroviral plasmid containing mOrange2 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Orange2

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS-nlstdTomato

Plasmid

#67693

Purpose

Tol1 expression plasmid with UAS-driven myc tagged nlsTdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

6xMTnlsTomato

Tags

6xMyc-NLS

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCY 3010-05

Plasmid

#36211

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

LEU2

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Cerulean-LEU2

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CTGA-R13-AKSRV

Plasmid

#63622

Purpose

CTGA-R13-AKSRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CTGA-R13-AKSRV RNAP

Expression

Bacterial

Mutation

V725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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TOPO-BC43

Plasmid

#84437

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC43

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pERB130

Plasmid

#58284

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Tags

GFP and mCherry

Expression

Mammalian

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCY 3090-02

Plasmid

#36231

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEpolylinker

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mCherry-hphMX4

Available Since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-spo5DSR

Plasmid

#48983

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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V37 pDNR MCS SA - Donor

Plasmid

#11690

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

July 19, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pERB127

Plasmid

#58283

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI)

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh
FKBP-targeted miRNA

Tags

GFP and mCherry

Expression

Mammalian

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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jk089_h12

Plasmid

#112151

Purpose

C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

C. thermophilum DASH/Dam1 complex

Tags

his tag on Spc34 and strep tag on Dad1

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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KpAvs4

Plasmid

#188796

Purpose

Avs4 from Klebsiella pneumoniae UNMC_8078

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDZ264 (pKAN tdtomato cyc loxP KAN loxP)

Plasmid

#35193

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

tdtomato cyc loxP KAN loxP

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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V1663 pLPS-3'RFP - Acceptor

Plasmid

#11701

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

RFP

Expression

Mammalian

Available Since

May 26, 2006

Availability

Academic Institutions and Nonprofits only

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V1662 pLP-RFP - Acceptor

Plasmid

#11700

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

RFP

Expression

Mammalian

Available Since

May 26, 2006

Availability

Academic Institutions and Nonprofits only

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pTol1-uas-mAKT-2a-nlstdTomato

Plasmid

#67706

Purpose

Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

mAKT-2A-nlstdTomato

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only