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177,692 results

Showing: 10801 - 10820 of 177692 results

  2. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  3. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70018-plasmid-map-sequence-id-124557

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  6. Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

    Plasmid
    #69623
    Purpose
    A FRET sensor for Galphai1 activation, encoded on a single plasmid.
    Depositor
    Dorus Gadella
    Publication
    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gbeta-2A-cpV-Ggamma2
    Galphai-mTurquoise2
    Tags
    ciruclar permutated Venus (cp173V) and mTurquoise…
    Expression
    Mammalian
    Promoter
    CMV and CMV (IRES)
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    69623-plasmid-map-sequence-id-459280

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  9. GPER-Tango

    Plasmid
    #66299
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPER (GPER1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    66299-plasmid-map-sequence-id-173899

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  10. CXCR2-Tango

    Plasmid
    #66260
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CXCR2 (CXCR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    66260-plasmid-map-sequence-id-344624

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  11. ADRA2A-Tango

    Plasmid
    #66216
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADRA2A (ADRA2A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66216-plasmid-map-sequence-id-417865

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  14. GalToxBFP

    Plasmid
    #68073
    Purpose
    mammalian expression of GC localized oxBFP
    Depositor
    Erik Snapp
    Publication
    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GalT signal anchor transmembrane domain
    Tags
    moxBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68073-plasmid-map-sequence-id-258728

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  18. pCMV-Tag4-OMA1

    Plasmid
    #61719
    Purpose
    mammalian expression of human Oma1 with C-terminal FLAG tag
    Depositor
    Oleh Khalimonchuk
    Insert
    OMA1 (OMA1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61719-plasmid-map-sequence-id-175330

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  20. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Publication
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65216-plasmid-map-sequence-id-115626

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Showing: 10801 - 10820 of 177692 results