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pDule-tfmF A65V S158A

Plasmid

#85484

Purpose

Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A

Tags

None

Expression

Bacterial

Mutation

Y32V F108W Q109M I159M with 65Val and 158Ala

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-mCl3-iLID

Plasmid

#190165

Purpose

Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-mClover3-iLID

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBluescript II KS(+) ACMV DNA-A 1.4mer

Plasmid

#159134

Purpose

Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-A

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

ACMV DNA-A 1.4mer

Use

Infectious clone for bombarding plants

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-mCherry

Plasmid

#75161

Purpose

Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

Plasmid

#223124

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pKDEL-mScarlet3-H (aka mYongHong)

Plasmid

#239411

Purpose

Expresses mScarlet3-H (aka mYongHong) localized to ER in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

mScarlet3-H-KDEL

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CuO-LacI-NLS–SNAPf

Plasmid

#227368

Purpose

Lentiviral expression of LacI–SNAP

Depositor

David Pellman

Article

Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

Insert

LacI NLS SNAPf

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald

Plasmid

#164210

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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NGFR

Plasmid

#27489

Depositor

Warren Pear

Article

Izon et al Immunity. 2001 Mar . 14(3):253-64.

Type

Empty backbone

Use

Retroviral

Tags

ECMV, IRES, and NGFR

Expression

Mammalian

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV2.5-THP-GFP

Plasmid

#80336

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Article

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Promoter

rat Tyrosine hydroxylase

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ATF4

Plasmid

#192911

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ATF4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ATF4 (ATF4 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN-MEF2C

Plasmid

#232627

Purpose

Lentiviral plasmid expressing mouse MEF2C.

Depositor

Claes Wadelius

Article

Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

Insert

MEF2C (Mef2c Mouse)

Use

Lentiviral

Tags

HA-3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCE-K2N

Plasmid

#154879

Purpose

Episomally expresses KLF2 and NANOG in mammalian cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

Insert

KLF2-2A-NANOG (KLF2, NANOG Human)

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Sumo1

Plasmid

#21154

Purpose

Mammalian expression of human Sumo1 with HA tag

Depositor

Junying Yuan

Article

Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

Insert

pcDNA3-HA-Sumo1 (SUMO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pMF440

Plasmid

#62550

Purpose

Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.

Depositor

Michael Franklin

Insert

mCherry

Expression

Bacterial

Promoter

trc

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-GRAM-W

Plasmid

#211704

Purpose

Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)

Depositor

Yasunori Saheki

Article

Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

Insert

EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Changed Glycine 187 to Tryptophan

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Lifeact-mCherry

Plasmid

#67302

Purpose

Fluorescent indicator for F-actin

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Lifeact-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Bmal1

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPGrp-Kras

Plasmid

#236001

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

InPGrp-Kras (Cyth3 Rat, Synthetic)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC29A1

Plasmid

#131927

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC29A1 (SLC29A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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