We narrowed to 67,501 results for: cel

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MBD_R44Q-IFP2.0C

Plasmid

#215749

Purpose

Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD-IFP2.0C

Plasmid

#215750

Purpose

Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-TEX264 LIR4A-FLAG

Plasmid

#128259

Purpose

Expresses TEX264 LIR4A in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

TEX264 LIR4A (TEX264 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed FEEL (aa 273-276) to alanines

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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doraWT_rescue_construct

Plasmid

#190609

Purpose

Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSlug-4

Plasmid

#40648

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-4 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pHR- flag-Lag16-fibcon-iCAM1

Plasmid

#205212

Purpose

This vector encodes of the synCAM (fibcon - iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM fibcon - iCAM1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PhLP1-myc-6xHis

Plasmid

#218176

Purpose

Expresses phosducin-like protein 1 in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

Phosducin like protein 1 (PDCL Human)

Tags

6x His and Myc

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-sfGFP

Plasmid

#116917

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

J23101

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1.miR16

Plasmid

#128747

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM22-Halo-FRB

Plasmid

#197059

Purpose

Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Rab5-Blasticidin

Plasmid

#120715

Purpose

Expresses early endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab5a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Amino acids 2-215

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_FRB-GEM23-Halo

Plasmid

#197060

Purpose

Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

I3-01

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCFP-LacI-NLS

Plasmid

#103840

Purpose

Flourescent marker for lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CFP-LacI-NLS

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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attB53-Pac-TRE-mCherry-attB53

Plasmid

#183611

Purpose

RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

TRE-mCherry

Use

Rmce

Promoter

TRE

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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3XFlag DVL2 (∂DIX)

Plasmid

#24805

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of the N-terminal DIX domain

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pLL3.7-K122-TEAD-p-H2B-mCherry

Plasmid

#139648

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

IRES-GFP

Use

Lentiviral

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR FOXP3

Plasmid

#16363

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Tags

Myc

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pLEX-FKBP-Rab11-Blasticidin

Plasmid

#120717

Purpose

Expresses recycling endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab11a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Amino acids 2-216

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-WIP

Plasmid

#11772

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP (WIPF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3puro-mGFP-FMR1iso1-G266E

Plasmid

#225468

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1 (G266E)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

G266E

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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